PMID: 375231Mar 1, 1979Paper

Replication in vitro of the 2-micrometer DNA plasmid of yeast

Proceedings of the National Academy of Sciences of the United States of America
S M Jazwinski, G M Edelman

Abstract

Cell-free extracts prepared from growing cells of the budding yeast Saccharomyces stimulated DNA synthesis directed by the supercoiled 2-micrometer yeast DNA plasmid. The major products of the reaction were open-circular daughter molecules possessing newly synthesized full-length linear DNA strands. Some of these were ligated and supertwisted by the extracts to yield a supercoiled DNA product. Both of the complementary DNA strands of the template were replicated. In addition, the extracts induced the appearance of theta-forms of the plasmid DNA, which are presumed to be replicative intermediates. The results of experiments utilizing BrdUTP incorporation indicated that DNA repair did not contribute significantly to the overall reaction. Extracts prepared from the cell division cycle mutants cdc7 and cdc8, held in culture at the nonpermissive temperature, possessed diminished activity. Because these mutants define a dependent sequence of events leading from the start of the cell cycle through G1 to S phase, this result suggests that the activity that stimulates 2-micrometer DNA replication in vitro is subject to control in the yeast cell cycle.

References

Jan 1, 1977·Nucleic Acids Research·J R CameronR W Davis
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S M Jazwinski, G M Edelman
Mar 1, 1975·Cell·T D Petes, D H Williamson
Jan 11, 1974·Science·L H HartwellB J Reid
Jan 16, 1974·European Journal of Biochemistry·G D Clark-Walker, G L Miklos
Apr 15, 1974·Journal of Molecular Biology·L M Hereford, L H Hartwell
Aug 1, 1971·Experimental Cell Research·J Culotti, L H Hartwell

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Citations

Jan 1, 1989·Experimental Gerontology·S M JazwinskiJ B Chen
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M TaketoG M Edelman
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H KojoA Sugino
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M Jazwinski, G M Edelman
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C KuoJ L Campbell
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J ArendesA Sugino
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Ishimi, K Matsumoto
Apr 1, 1986·Experimental Cell Research·M M Hurt, R E Moses
Sep 16, 1980·Biochemical and Biophysical Research Communications·K Nakayama, A Sugino
Feb 1, 1987·Biotechnology and Bioengineering·C Parker, D Dibiasio
Oct 6, 2005·Physical Biology·Vladislav VolmanEshel Ben-Jacob
Aug 1, 1981·European Journal of Biochemistry·G BadaraccoP Plevani
Dec 1, 1988·Microbiological Reviews·C S Newlon
Jan 1, 1984·Molecular and Cellular Biology·M S Whiteway, A Ahmed
Apr 1, 1984·Molecular and Cellular Biology·L G BirkenmeyerL B Dumas

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