Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis

Differentiation; Research in Biological Diversity
L EtkinS L Bektesh

Abstract

We have analyzed the fate of circular and linear DNA molecules following microinjection into the cytoplasm of fertilized eggs of Xenopus laevis. Recombinant plasmids containing sea urchin histone genes (pSp 102), Drosophila ADH genes (sAC-1), and SV40 (SV2 CAT) replicate during the development of the injected frog embryo. In contrast, pBR322 either as monomers or multimers does not appear to replicate as efficiently. Generally, injected circular DNAs were not detectable by the gastrula stage of development, although there were several examples in which these molecules persisted until larval stages. In 90% of the cases, injected linear DNAs persisted as discrete molecules into early embryonic stages. A portion of the DNA sequences complementary to injected linear and circular molecules was detected comigrating with the high-molecular-weight cellular frog DNA (48 kb or larger) from mid-cleavage stages onward. Restriction enzyme analysis of DNA from injected embryos suggested some copies of the injected DNAs were integrated into the frog genome. This occurred in about 10%-30% of the cases of injected circular DNA and approximately 60%-70% of the cases of injected linear DNA. We were able to rescue circular plasmids from the inject...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Nov 1, 1975·Cell·L H KedesS N Cohen
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Feb 1, 1980·Cell·E A FyrbergK L Kindle
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R M Harland
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·G GargiuloA Worcel
Dec 1, 1983·Molecular and Cellular Biology·N C JonesL D Smith
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M M Bendig, J G Williams
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A Goldberg
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C BenyajatiW Sofer
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Rusconi, W Schaffner
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S HiragaH Ueyama
Jan 1, 1981·Annual Review of Genetics·J B Gurdon, D A Melton

❮ Previous
Next ❯

Citations

Apr 1, 1991·Molecular Reproduction and Development·M KlocL D Etkin
Sep 15, 1991·Experientia·L M Houdebine, D Chourrout
Aug 1, 1990·Trends in Biotechnology·T T Chen, D A Powers
Aug 14, 2008·Biology of the Cell·Albert ChesneauOdile J Bronchain
Nov 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Fong Cheng PanTomas Pieler
May 4, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Erin KaltenbrunFrank L Conlon
Nov 16, 2011·Genesis : the Journal of Genetics and Development·Margaret B FishRobert M Grainger
Oct 1, 1988·Experimental Cell Research·M J Mancebo, L D Etkin
Apr 1, 1987·Experimental Cell Research·L D EtkinR Ansah-Yiadom
Jan 1, 1989·Molecular Reproduction and Development·H Sang, M M Perry
Jan 13, 2000·Differentiation; Research in Biological Diversity·S G Gong, A Kiba
Jan 1, 1992·Differentiation; Research in Biological Diversity·R S WinningJ J Heikkila
Sep 30, 1991·Biochemical and Biophysical Research Communications·B A ReddyL D Etkin
Oct 23, 1997·Developmental Biology·H M El-HodiriL D Etkin
Jan 18, 2006·Mechanisms of Development·Hajime OginoRobert M Grainger
Nov 1, 1991·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Margaret PerryHelen Sang
Mar 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Koichiro ShiokawaKeiichi Hosokawa
Aug 1, 1987·Molecular and Cellular Biology·L DesGroseillersR H Scheller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.