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Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data

Molecular Ecology Resources

Oct 21, 2014

Gentile Francesco FicetolaPierre Taberlet

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Abstract

Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and past biodiversity. eDNA analyses often rely on amplification of very small quantities or degraded DNA. To avoid missing detection of taxa that are actually present (false negatives), multiple extraction...read more

Mentioned in this Paper

Gene Amplification Technique
Sequencing
Gene Amplification Abnormality
Observation - Diagnostic Procedure
14
598
Paper Details
References
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123
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Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data

Molecular Ecology Resources

Oct 21, 2014

Gentile Francesco FicetolaPierre Taberlet

PMID: 25327646

DOI: 10.1111/1755-0998.12338

Abstract

Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and past biodiversity. eDNA analyses often rely on amplification of very small quantities or degraded DNA. To avoid missing detection of taxa that are actually present (false negatives), multiple extraction...read more

Mentioned in this Paper

Gene Amplification Technique
Sequencing
Gene Amplification Abnormality
Observation - Diagnostic Procedure
14
598

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

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Examining the genomics, transcriptomics, proteomics and metabolomics of the human vascular endothelial cells at the single cell level will help build a single cell atlas of human vascular endothelium, which would give a unique ID card for each cell type, a three-dimensional map of how cell types work together to form tissues and insights into how changes in the map underlie health and disease of the vascular endothelium from young to old age. Disocver the latest research on the single cell atlas of human vascular endothelial cell atlas here.

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Paper Details
References
  • References26
  • Citations68
123
  • References26
  • Citations68
12345...

Download from

Publisher
PubMed
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