PMID: 2506591May 1, 1989Paper

Replication of an RK2 miniplasmid derivative in vitro by a DNA/membrane complex extracted from Escherichia coli: involvement of the dnaA but not dnaK host proteins and association of these and plasmid-encoded proteins with the inner membrane

Plasmid
D A KostyalW Firshein

Abstract

A DNA/membrane complex extracted from a miniplasmid derivative of the broad host range plasmid RK2 cultured in Escherichia coli capable of synthesizing new plasmid supercoiled DNA in vitro was treated with antibodies that were made against or reacted with the dnaA and dnaK host-encoded proteins, respectively. Anti-dnaA protein antibody inhibited total plasmid DNA synthesis significantly and the synthesis of supercoil plasmid DNA almost completely. In contrast, anti-dnaK protein antibody and nonimmune serum had little or no effect on total plasmid DNA synthesis. Both proteins were found to be present in the inner but not outer membrane fraction of E. coli. A variety of miniplasmid-encoded proteins which had previously been found in the DNA/membrane complex have also been localized to the inner but not outer membrane fraction. These include an essential initiation protein of 32 kDa (and an overlapping protein of 43 kDa coded for by the same gene), as well as a 30-kDa protein that may be linked to incompatibility functions. Various extraction methods were used to distinguish between the associated and the integral nature of the plasmid-encoded proteins. The results demonstrated that the essential replication proteins (32 and 43 kD...Continue Reading

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Citations

Dec 20, 1996·Biotechnology and Bioengineering·H Kuo, J D Keasling
Nov 24, 1999·FEMS Microbiology Letters·D E LopatinC E Shelburne
Sep 1, 1990·Molecular Microbiology·M Motallebi-VesharehC M Thomas
Jan 18, 2005·FEMS Microbiology Reviews·Alicia BravoMargarita Salas
Apr 3, 1999·FEMS Microbiology Letters·J JyotA Ghosh
Jan 10, 1998·Infection and Immunity·M HarboeS Nagai
Nov 1, 1995·Journal of Bacteriology·F WuM Filutowicz
Jul 1, 1991·Journal of Bacteriology·J Meury, M Kohiyama
Jul 6, 2010·Plasmid·Katarzyna KolatkaIgor Konieczny

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