Replication of classical infectious bursal disease virus in the chicken embryo related cell line

Avian Pathology : Journal of the W.V.P.A
T C CardosoC W Arns

Abstract

Infectious bursal disease (IBD) is an acute, highly contagious viral disease. The diagnosis of IBD depends on time-consuming and costly procedures, like virus isolation on chick embryos and histopathological examination. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), immunoperoxidase and reverse transcription polymerase chain reaction (RT-PCR) were applied in this study to detect classical IBD virus (IBDV) after three blind passages of the Lukert strain on chicken embryo related (CER) cell monolayer after different periods of infection: 6, 12, 24 and 48h. Cytophatic effects were most evident 12 h post-infection (p.i.) but were observed at 6 h p.i. The maximum discrimination between IBDV-infected and uninfected cell suspensions obtained by the use of DAS-ELISA for virus detection corresponded to 0.597 +/-0.02 and 0.010 +/-0.01 after 12 h p.i., respectively. The RT-PCR was performed using the set of primers A3.1 and A3.2 to amplify the VP2 region of the IBDV genome. This molecular technique demonstrated that from 6h p.i., it was possible to detect the viral RNA. The results show that the CER cell line can be used for classical IBDV propagation, confirmed by the DAS-ELISA, immunoperoxidase and RT-PCR assay.

References

Jan 1, 1977·Intervirology·A L SmithT Motohashi
May 1, 1991·The British Veterinary Journal·A Kumar, A T Rao
Aug 1, 1988·The Journal of General Virology·F S KibengeR G Russell
May 17, 2008·Avian Pathology : Journal of the W.V.P.A·J O Okoye, E P Aba-Adulugba
May 17, 2008·Avian Pathology : Journal of the W.V.P.A·O L NielsenP H Jørgensen

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Citations

Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg
Jun 26, 2007·Avian Pathology : Journal of the W.V.P.A·Helena L FerreiraTereza Cardoso
Jul 16, 2008·Avian Pathology : Journal of the W.V.P.A·Tereza C CardosoMaria Cecilia R Luvizotto
Mar 12, 2010·Journal of Virological Methods·Lia Treptow CoswigClarice Weis Arns
Sep 21, 2007·Journal of Comparative Pathology·A R MendesT C Cardoso
Sep 27, 2005·Journal of Virological Methods·Fernando Rosado SpilkiClarice Weis Arns

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