Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli.

Journal of Bacteriology
M Abe

Abstract

The replication of ColE1 deoxyribonucleic acid (DNA) took place at the restrictive temperature in a dnaA mutant, dnaA167(Ts). It proceeded at a constant rate at 42 degrees C for at least 3 h. The replication was insensitive to rifampin, which blocked replication at the permissive temperature or in the presence of chloramphenicol, even at the restrictive temperature. A linear DNA strand of ColE1 longer than unit genome size was synthesized. The structure of the replicating molecules observed by electron microscopy was mostly sigma shaped, composed of a circle of a unit genome length with a double-stranded tail. These observations suggest that the replication of ColE1 DNA proceeds via a rolling-circle type of structure in the absence of dnaA function.

References

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·P Polaczek, Z Cieśla
Mar 1, 1991·Molecular & General Genetics : MGG·C S ChiangH Bremer
Jan 1, 1984·CRC Critical Reviews in Biochemistry·K J Marians

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