PMID: 4883017Nov 1, 1968Paper

Replication of coliphage M-13. II. Intracellular deoxyribonucleic acid forms associated with M-13 infection of mitomycin C-treated cells

Journal of Virology
L R Brown, C E Dowell

Abstract

Intracellular deoxyribonucleic acid (DNA) forms associated with bacteriophage M-13 infection have been isolated and characterized. Escherichia coli HF4704 (F(+), hcr(-), thy(-)) cells were treated with mitomycin C to inhibit host-cell DNA synthesis and were then infected with phage M-13. This treatment permitted radioactive labeling of phage-specific DNA forms with (3)H-thymine. These labeled DNA components were characterized by sucrose density sedimentation and equilibrium density gradient centrifugation in neutral and ethidium bromide CsCl gradient. Two double-stranded circular forms were found with properties analogous to the replicative form I and replicative form II of phiX174. A third component, identified as single-stranded DNA, was isolated in some samples removed 45 min after phage synthesis was initiated.

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Citations

Dec 1, 1975·CRC Critical Reviews in Microbiology·D T Denhardt
Jun 1, 1969·Bacteriological Reviews·D A Marvin, B Hohn
Jan 1, 1974·Journal of Virology·J M Bowes, C E Dowell

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