PMID: 2493706Mar 1, 1989Paper

Replication of recombinant phi 29 DNA molecules in Bacillus subtilis protoplasts

Virology
C EscarmísM Salas

Abstract

Recombinant phi 29 DNA molecules of different sizes and containing terminal protein at one or both ends, or without terminal protein, were prepared and their replication in Bacillus subtilis protoplasts was studied. Only phi 29 DNA molecules containing terminal protein at both ends replicate in vivo. The replication of symmetric DNA recombinant molecules (dimers) gives rise to displaced strands which by self-annealing create monomers with the two DNA strands covalently linked. Viral proteins p2, p3, and p6 are essential for replication of phi 29 DNA molecules in this system. Protein p17 is not essential, but stimulates the efficiency of replication. This stimulation depends on the host used.

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Citations

Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Isabel HolgueraMargarita Salas
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas

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