PMID: 518301Jan 1, 1979Paper

Replication of standard and defective Ross River virus in BHK cells: patterns of viral RNA and polypeptide synthesis

Archives of Virology
J H MartinL Dalgarno

Abstract

Virus-specific macromolecule synthesis has been examined in BHK cells infected with Ross River virus. Unpassaged virus (R-0) and tenth-passage virus (R-10) have been compared. In infected cells R-0 generates i) 45S, 28S, 33S and 26S viral RNAs, ii) virus-specific precursor polypeptides of mol. wt. 127,000, 95,000 and 61,000 and iii) viral envelope proteins (mol. wts. 52,000 and 49,000) and nucleocapsid protein (mol. wt. 32,000). Thus in terms of virus-specific RNA and polypeptide synthesis, the replication of standard RRV is analogous to that of Semliki Forest virus and Sindbis virus. R-10 interferes with the replication of standard Ross River virus and generates large amounts of 19S and 24S defective RNA species; 45S and 26S RNA synthesis was not markedly affected. Defective RNAs are associated with RNAse-sensitive, 50S cytoplasmic particles which contain a variety of (mainly host) proteins but no nucleocapsid protein. No evidence for translation of defective RNAs was obtained. R-10 infection is also characterized by a relatively early shut down of host protein syntehsis and by a reduction in virus-specific polypeptide synthesis and nucleocapsid formation. The data suggest that defective Ross River virus interferes primarily a...Continue Reading

References

Jun 1, 1976·The Journal of General Virology·C J BrutonS I Kennedy
Sep 1, 1976·Journal of Virology·S I KennedyS Schlesinger
Jan 1, 1973·Annual Review of Microbiology·A S Huang
Jan 1, 1974·Medical Microbiology and Immunology·S SchlesingerR Cancedda
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Feb 1, 1973·The Journal of Infectious Diseases·F A MurphyI D Marshall
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Sep 1, 1974·Journal of Virology·D T Simmons, J H Strauss
Nov 1, 1969·Journal of Virology·E G Westaway, B M Reedman
May 1, 1972·Virology·S SchlesingerB W Burge

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