Replication of synthetic defective interfering RNAs derived from coronavirus mouse hepatitis virus-A59

Virology
W LuytjesW J Spaan

Abstract

We have analyzed the replication of deletion mutants of defective interfering (DI) RNAs derived from the coronavirus mouse hepatitis virus (MHV)-A59 in the presence of MHV-A59. Using two parental DI RNAs, MIDI and MIDI delta H, a twin set of deletion mutants was generated with progressively shorter stretches of 5' sequence colinear with the genomic RNA. All deletion mutants contained in-frame ORFs. We show that in transfected cells and after one passage the DI RNAs were detectable and that their accumulation was positively correlated with the length of 5' sequence they contained. However, accumulation of two twin mutants, delta 2, in which sequences from nucleotide position 467 were fused to those from position 801, was undetectable. In passage 4 cells, but not in transfected or in passage 1 cells, recombination with genomic RNA led to the appearance of the parental DI RNAs. The accumulation of these parental RNAs was inversely correlated with the length of 5' sequence on the deletion mutants and was highest in the delta 2 samples. In sharp contrast to the data reported for MHV-JHM-derived DI RNAs, we show that MHV-A59-derived mutant RNAs do not require an internal sequence domain for replication. The data suggest that coronavi...Continue Reading

Citations

Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
Mar 5, 2015·Virus Research·Dong Yang, Julian L Leibowitz
Oct 14, 2014·Virus Research·Ramakanth MadhugiriJohn Ziebuhr
Dec 1, 2009·Viruses·Kunj B Pathak, Peter D Nagy
Mar 27, 2015·Journal of Virology·Kelley R Hurst-HessPaul S Masters
Jul 28, 2020·Biomolecules·Ken AsadaRyuji Hamamoto
Dec 3, 2020·Nucleic Acids Research·Sylvain de BreyneThéophile Ohlmann
Apr 25, 2020·Cell·Dongwan KimHyeshik Chang

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