PMID: 6991883Apr 1, 1980Paper

Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication

Molecular & General Genetics : MGG
A OkaY Hirota

Abstract

A DNA fragment containing the replication origin of the Eschericia coli K-12 chromosome was inserted in two correlations at either the BamHI or SalI site of pBR322 DNA. All the resulting hybrid plasmids were found to replicate in both polA and polA+ cells, whereas pBR322 replicates only in polA+ cells. This characteristic provided a method for assaying the autonomously replicating ability (Ori function of the E. coli origin. In order to define the minimum DNA region (ori) that determines Ori function, deletions of various sizes were introduced from either side of the ori-containing segment in the hybrid plasmids by in vitro techniques, and the correlation between the Ori phenotype and nucleotide sequence of the deletion derivatives was analyzed. It was found that the left end of ori is between positions 23 and 35, and the right end is either position 266 or 267 in our nucleotide coordinate (Sugimoto et al., 1979). Therefore, ori is present within a region of minimum 232 base pairs and maximum 245 base pairs in length. The Ori+ and Ori- phenotypes were clearly resolved at both sides of these boundaries by the above assay procedure. To obtain information about the effect of mutations in the internal region of the defined ori stre...Continue Reading

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Citations

Mar 1, 1996·Journal of Cellular Biochemistry·T Boulikas
Jan 1, 1990·Journal of Basic Microbiology·D J SavicT Kostic
Apr 1, 1986·Molecular & General Genetics : MGG·D E JunkerJ W Zyskind
Apr 1, 1989·Molecular & General Genetics : MGG·A LandoulsiM Kohiyama
Jan 1, 1985·Molecular & General Genetics : MGG·M Tanaka, S Hiraga
Aug 1, 1986·Molecular & General Genetics : MGG·M YoshimotoG Tamura
Jan 1, 1992·Folia Microbiologica·J Hubácek
Jan 1, 1985·Folia Microbiologica·J Nesvera, J Hochmannová
Jul 15, 1984·Journal of Molecular Biology·A OkaM Takanami
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S L ZipurskyJ Hurwitz
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R S FullerA Kornberg
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M SeikiH Yoshikawa
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K YamaguchiJ Tomizawa
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Tsurimoto, K Matsubara
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R S Fuller, A Kornberg
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J W ZyskindD W Smith
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T OgawaA Kornberg
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A van der EndeA Kornberg
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Jun 10, 1988·Nucleic Acids Research·H YamakiY Maeda

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