Replication signals in the genome of vesicular stomatitis virus and its defective interfering particles: identification of a sequence element that enhances DI RNA replication

Virology
T Li, A K Pattnaik

Abstract

We have analyzed the role of terminal sequences of a defective interfering (DI) particle RNA of vesicular stomatitis virus (VSV) in replication. A series of internal deletion mutants of DI cDNA was generated to obtain DI genomic RNAs that differed from one another by the presence of different lengths of 3'-terminal and/or 5'-terminal sequences. Analyses of the mutant. RNAs for their ability to replicate in cells transfected with the corresponding plasmids suggested that distinct regions at the termini of DI RNA are important for RNA replication. Region I, encompassing nucleotides 1-24, is absolutely required for replication since DI RNA genomes lacking any part of this region failed to replicate. Region II, spanning nucleotides 25-45, is not essential for replication but it functions as an enhancer of replication in that the presence of these specific sequences confers high efficiency of replication to the template. Deleting these specific sequences from both termini of DI RNA but maintaining the length of terminal complementarity as seen in wild-type DI RNA resulted in a template that replicated poorly (about 20-fold less efficiently). Furthermore, insertion or substitution of these sequences into the 3'-terminus of a VSV mini...Continue Reading

References

Jul 1, 1975·Virology·D Kolakofsky, A Bruschi
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A K Pattnaik, G W Wertz
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
May 1, 1988·Molecular and Cellular Biology·R L Feinbaum, F M Ausubel
Mar 1, 1987·Microbiological Reviews·A K Banerjee
Jan 5, 1974·Journal of Molecular Biology·R N Leamnson, M E Reichmann
Sep 1, 1974·Cell·G Stamminger, R A Lazzarini
Apr 25, 1970·Nature·A S Huang, D Baltimore
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J D KeeneS U Emerson
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P WhelanG T Wertz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·G W WertzL A Ball
Feb 15, 1996·Virology·E KretzschmarJ K Rose

❮ Previous
Next ❯

Citations

Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sean P J Whelan, Gail W Wertz
Nov 21, 2001·The Journal of General Virology·V MiouletM D Baron
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Sep 19, 2003·The Journal of General Virology·Elizabeth R ChareEdward C Holmes
Jun 5, 2019·Nature Microbiology·Marco Vignuzzi, Carolina B López
Feb 16, 2006·The Journal of General Virology·Samuel Cordey, Laurent Roux
Sep 6, 2002·Biochimica Et Biophysica Acta·John N BarrGail W Wertz

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.