Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase.

Proceedings of the National Academy of Sciences of the United States of America
D BastiaMarc M Greenberg

Abstract

The replication terminator protein Tus of Escherichia coli promotes polar fork arrest at sequence-specific replication termini (Ter) by antagonizing DNA unwinding by the replicative helicase DnaB. Here, we report that Tus is also a polar antitranslocase. We have used this activity as a tool to uncouple helicase arrest at a Tus-Ter complex from DNA unwinding and have shown that helicase arrest occurred without the generation of a DNA fork or a bubble of unpaired bases at the Tus-Ter complex. A mutant form of Tus, which reduces DnaB-Tus interaction but not the binding affinity of Tus for Ter DNA, was also defective in arresting a sliding DnaB. A model of polar fork arrest that proposes melting of the Tus-Ter complex and flipping of a conserved C residue of Ter at the blocking but not the nonblocking face has been reported. The model suggests that enhanced stability of Tus-Ter interaction caused by DNA melting and capture of a flipped base by Tus generates polarity strictly by enhanced protein-DNA interaction. In contrast, the observations presented here show that polarity of helicase and fork arrest in vitro is generated by a mechanism that not only involves interaction between the terminator protein and the arrested enzyme but a...Continue Reading

References

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Feb 16, 2006·Journal of the American Chemical Society·In Seok HongMarc M Greenberg

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Citations

Mar 21, 2009·Molecular Microbiology·Daniel L Kaplan, Deepak Bastia
Nov 21, 2008·Molecular Microbiology·Iain G DugginThomas M Hill
Dec 9, 2014·Cell Cycle·Nicolai B LarsenHocine W Mankouri
May 28, 2014·FEMS Microbiology Letters·Valentin V Rybenkov
Jul 7, 2015·Nature Chemical Biology·Jun Fan, Terence R Strick
Jul 7, 2015·Nature Chemical Biology·Bojk A BerghuisNynke H Dekker
May 27, 2015·Nucleic Acids Research·Manjula PandeySmita S Patel
Jun 23, 2015·Frontiers in Microbiology·Thomas R Beattie, Rodrigo Reyes-Lamothe
Aug 26, 2011·Bioarchitecture·Deepak Bastia, Samarendra K Singh
Feb 18, 2011·Transcription·James Byrnes, Miguel Garcia-Diaz
May 12, 2016·Nucleic Acids Research·M Charl MoolmanNynke H Dekker
May 9, 2014·Seminars in Cell & Developmental Biology·Deepak Bastia, Shamsu Zaman
May 18, 2016·Chemistry : a European Journal·Yibin WangXiaohua Peng
Oct 27, 2017·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Nov 8, 2017·Critical Reviews in Biochemistry and Molecular Biology·Bojk A BerghuisNynke H Dekker
Apr 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Rahul JaiswalCarlos R Escalante
May 30, 2017·DNA Repair·Michael A TrakselisRobert M Brosh
Nov 6, 2020·Chemical Research in Toxicology·Shane R Byrne, Steven E Rokita
Jun 16, 2021·The EMBO Journal·Daniel González-AcostaJuan Méndez
Nov 16, 2021·The Journal of Biological Chemistry·Katie H JamesonMichelle Hawkins

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