Replication-transcription conflicts trigger extensive DNA degradation in Escherichia coli cells lacking RecBCD

DNA Repair
Juachi U DimudeChristian J Rudolph

Abstract

Bacterial chromosome duplication is initiated at a single origin (oriC). Two forks are assembled and proceed in opposite directions with high speed and processivity until they fuse and terminate in a specialised area opposite to oriC. Proceeding forks are often blocked by tightly-bound protein-DNA complexes, topological strain or various DNA lesions. In Escherichia coli the RecBCD protein complex is a key player in the processing of double-stranded DNA (dsDNA) ends. It has important roles in the repair of dsDNA breaks and the restart of forks stalled at sites of replication-transcription conflicts. In addition, ΔrecB cells show substantial amounts of DNA degradation in the termination area. In this study we show that head-on encounters of replication and transcription at a highly-transcribed rrn operon expose fork structures to degradation by nucleases such as SbcCD. SbcCD is also mostly responsible for the degradation in the termination area of ΔrecB cells. However, additional processes exacerbate degradation specifically in this location. Replication profiles from ΔrecB cells in which the chromosome is linearized at two different locations highlight that the location of replication termination can have some impact on the degr...Continue Reading

Citations

Dec 14, 2018·Nucleic Acids Research·Sarah L Midgley-SmithChristian J Rudolph
Apr 15, 2020·FEMS Microbiology Reviews·Anurag Kumar SinhaDavid R F Leach
Mar 15, 2019·Nucleic Acids Research·Michelle HawkinsPeter McGlynn
May 1, 2020·Frontiers in Microbiology·Aisha H SyedaChristian J Rudolph
Mar 19, 2019·Molecular Microbiology·Nicklas A HamiltonJustin Courcelle

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