Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis

Plasmid
Juan A Rojas ContrerasAntonio De León-Rodríguez

Abstract

Integrative and replicative plasmids for the expression driven by the P(43) promoter and secretion of recombinant proteins in Bacillus subtilis were constructed. The plasmids named pInt and pRep respectively were tested for the production of recombinant human interferon gamma (rhIFN-γ). A synthetic hIFN-γ gene employing the optimized B. subtilis codon usage was fused with the Bacillus licheniformis α-amylase signal peptide (sp-amyL) encoding sequence. The integrative construct produced 2.5±0.2mgl(-1) and the replicative system produced 20.3±0.8mgl(-1) of total recombinant rhIFN-γ. The results showed that secretion of hIFN-γ was the bottleneck for the overexpression of mature rhIFN-γ by B. subtilis.

References

Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D G Yansura, D J Henner
Aug 5, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P R MadyasthaL L Key
Mar 10, 2001·Applied Microbiology and Biotechnology·J JanE Merino
Feb 11, 2003·Microbiology·Leendert W HamoenOscar P Kuipers
Jul 13, 2004·Trends in Biotechnology·Claes GustafssonJeremy Minshull
Dec 20, 2005·Journal of Biotechnology·Lidia WestersWim J Quax
Mar 11, 2008·Protein Expression and Purification·Victor E Balderas HernándezAntonio De León Rodríguez
Mar 13, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takuya MorimotoNaotake Ogasawara
Feb 19, 2009·Biotechnology Progress·Tunçer H OzdamarPinar Calik
Sep 3, 2009·Journal of Agricultural and Food Chemistry·Hsin-Hung LinShann-Tzong Jiang

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Citations

Oct 17, 2017·Applied and Environmental Microbiology·Sakshi KhosaLutz Schmitt
Sep 12, 2018·World Journal of Microbiology & Biotechnology·Wenjing CuiZhemin Zhou
Apr 25, 2018·Preparative Biochemistry & Biotechnology·Nitin KumarVeeranki Venkata Dasu
Aug 24, 2019·Analytical Biochemistry·Reza Heidari-JapelaghiMokhtar Jalali-Javaran

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