PMID: 9449539Feb 4, 1998Paper

Replicative characteristics of primary isolates of the human immunodeficiency virus type 1 in peripheral blood mononuclear cells, primary macrophages and CD4+ transformed T-cell lines

Cellular and Molecular Biology
P E Jolly

Abstract

Macrophage-tropic strains of HIV-1 are selectively transmitted and play a significant role in HIV-1 persistence, dissemination and disease progression. Most primary HIV-1 isolates productively infect primary macrophages but fail to infect or induce syncytium (non-syncytium-inducing or NSI) in CD4+ transformed T-cell lines. Therefore, NSI isolates are considered to be macrophage-tropic. In order to select truly macrophage-tropic primary HIV-1 isolates for pathogenesis studies the replicative characteristics of a panel of eight primary (predominantly NSI) HIV-1 isolates were examined in primary macrophage cultures. This was not previously done. Stocks of these isolates were prepared in peripheral blood mononuclear cells (PBMC) and their replication in peripheral blood-derived macrophages and in CD4+ transformed T-cell lines examined based on the p24 antigen level in the cultures. Seven of the eight isolates did not replicate or induce syncytia (non-syncytium-inducing or NSI) in MT-2 cells, but one isolate replicated efficiently and induced large syncytia (syncytium-inducing or SI) in both the MT-2 and another transformed T-cell line (CEMx174). When replication of the isolates in peripheral blood-derived macrophages was examined, ...Continue Reading

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