Repopulation dynamics of single haematopoietic stem cells in mouse transplantation experiments: Importance of stem cell composition in competitor cells

Journal of Theoretical Biology
H EmaYoh Iwasa

Abstract

The transplantation of blood tissues from bone marrow into a lethally irradiated animal is an experimental procedure that is used to study how the blood system is reconstituted by haematopoietic stem cells (HSC). In a competitive repopulation experiment, a lethally irradiated mouse was transplanted with a single HSC as a test cell together with a number of bone marrow cells as competitor cells, and the fraction of the test cell progeny (percentage of chimerism) was traced over time. In this paper, we studied the stem cell kinetics in this experimental procedure. The balance between symmetric self-renewal and differentiation divisions in HSC determined the number of cells which HSC produce and the length of time for which HSC live after transplantation. The percentage of chimerism depended on the type of test cell (long-, intermediate-, or short-term HSC), as well as the type and number of HSC included in competitor cells. We next examined two alternative HSC differentiation models, one-step and multi-step differentiation models. Although these models differed in blood cell production, the percentage of chimerism appeared very similar. We also estimated the numbers of different types of HSC in competitor cells. Based on these re...Continue Reading

References

Feb 1, 1990·Genes & Development·C T Jordan, I R Lemischka
Apr 1, 1972·Journal of Cellular Physiology·H S MicklemD S Papworth
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S H CheshierI L Weissman
Nov 9, 2000·The Journal of Experimental Medicine·K SudoH Nakauchi
May 9, 2001·International Reviews of Immunology·Y Katsura, H Kawamoto
Apr 5, 2007·Nature Protocols·Hideo EmaHiromitsu Nakauchi
Mar 29, 2008·Cell Stem Cell·Brad DykstraConnie Eaves
Apr 7, 2009·Biochemical and Biophysical Research Communications·Shinichi NodaHiroyuki Miyoshi
Dec 4, 2009·Nature·Thomas Graf, Tariq Enver
Mar 9, 2010·Cell Stem Cell·Grant A ChallenMargaret A Goodell
Apr 28, 2010·The Journal of Experimental Medicine·Yohei MoritaHiromitsu Nakauchi
Feb 9, 2011·The Journal of Experimental Medicine·Hitoshi TakizawaMarkus G Manz
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hans B SieburgChrista E Muller-Sieburg
Mar 13, 2012·Blood·Christa E Muller-SieburgGiulio Cattarossi
Nov 26, 2013·Experimental Hematology·Hideo EmaToshio Suda
Apr 22, 2014·Stem Cell Reports·Jiajing QiuKateri Moore

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