Representational difference analysis identifies specific genes in the interaction of Giardia duodenalis with the murine intestinal epithelial cell line, IEC-6

International Journal for Parasitology
Showgy Yasir Ma'ayeh, Phillip Thomas Brook-Carter

Abstract

Giardia duodenalis is a re-emerging protozoan parasite that causes diarrhoea in humans, significantly affecting the health of many people globally. To date, little is known about the genetic events underpinning the establishment of infection in host cells; however, the parasite's ventral disc, proteases and variable surface proteins (VSPs) are recognised as important pathogenic factors. In this study, representational difference analysis (RDA) was used to identify differentially expressed genes in four different Giardia isolates (WB, P-1, NF and GS/M) during the first 2h of in vitro interaction with the rat intestinal epithelial cell line, IEC-6. RDA showed that more than 40 genes were differentially expressed in each of the four Giardia isolates upon IEC-6 cells infection. Most of the up-regulated genes were common to the four isolates except for those encoding proteins possibly involved in immune evasion such as VSPs, high cysteine membrane proteins (HCMp), hypothetical proteins, and oxygen defence proteins (e.g., thioredoxin, peroxiredoxin 1). Differences in the expressed VSPs and HCMp may account for the variation in symptoms during giardiasis. Interestingly, the NF isolate solely expressed genes involved in encystation dur...Continue Reading

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Citations

Jan 15, 2014·PLoS Neglected Tropical Diseases·Daniela MastronicolaAlessandro Giuffrè
Dec 18, 2015·Molecular and Biochemical Parasitology·Daniela MastronicolaAlessandro Giuffrè
Sep 6, 2015·International Journal for Parasitology·Showgy Y Ma'ayehStaffan G Svärd
Apr 30, 2015·Biotechnology Advances·Brendan R E AnsellAaron R Jex
Oct 7, 2014·Molecular and Biochemical Parasitology·Marcela FerellaStaffan Svärd
Jul 31, 2013·Experimental Parasitology·Juri KimSoon-Jung Park
Jun 18, 2017·Frontiers in Cellular and Infection Microbiology·Jonathan K PhamScott C Dawson
Apr 30, 2017·Tissue Barriers·Thibault AllainAndré G Buret
Dec 5, 2015·PLoS Neglected Tropical Diseases·Brendan R E AnsellAaron R Jex
Oct 6, 2020·Frontiers in Genetics·Dimitra PeirasmakiStaffan G Svärd
Jul 8, 2019·Trends in Parasitology·Thibault AllainAndre G Buret

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