Repression and derepression of minus-strand synthesis in a plus-strand RNA virus replicon

Journal of Virology
Guohua ZhangA E Simon

Abstract

Plus-strand viral RNAs contain sequences and structural elements that allow cognate RNA-dependent RNA polymerases (RdRp) to correctly initiate and transcribe asymmetric levels of plus and minus strands during RNA replication. cis-acting sequences involved in minus-strand synthesis, including promoters, enhancers, and, recently, transcriptional repressors (J. Pogany, M. R. Fabian, K. A. White, and P. D. Nagy, EMBO J. 22:5602-5611, 2003), have been identified for many viruses. A second example of a transcriptional repressor has been discovered in satC, a replicon associated with turnip crinkle virus. satC hairpin 5 (H5), located proximal to the core hairpin promoter, contains a large symmetrical internal loop (LSL) with sequence complementary to 3'-terminal bases. Deletion of satC 3'-terminal bases or alteration of the putative interacting bases enhanced transcription in vitro, while compensatory exchanges between the LSL and 3' end restored near-normal transcription. Solution structure analysis indicated that substantial alteration of the satC H5 region occurs when the three 3'-terminal cytidylates are deleted. These results indicate that H5 functions to suppress synthesis of minus strands by sequestering the 3' terminus from th...Continue Reading

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Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaobing ZuoYun-Xing Wang
Dec 25, 2010·The Journal of Biological Chemistry·Claudia V FilomatoriAndrea V Gamarnik
Jun 27, 2008·Journal of Virology·John C McCormackAnne E Simon
Jun 29, 2004·Journal of Virology·John C McCormack, Anne E Simon
Jul 30, 2005·Journal of Virology·Zivile PanavienePeter D Nagy
May 21, 2013·Annual Review of Microbiology·Anne E Simon, W Allen Miller
May 18, 2006·Annual Review of Phytopathology·W Allen Miller, K Andrew White
Oct 29, 2010·Virus Research·Aurora CastañoCarmen Hernández
Sep 19, 2015·Nucleic Acids Research·Peter AshtonK Andrew White
Mar 13, 2014·Virology·Pui Kei K Lee, K Andrew White
Mar 8, 2011·Virology·Joanna Sztuba-SolińskaJozef J Bujarski
Feb 11, 2015·Virus Research·Anne E Simon
Mar 12, 2015·Virology·Laura R Newburn, K Andrew White
Aug 16, 2008·Virus Research·Sergio M Villordo, Andrea V Gamarnik
Dec 21, 2005·Virology·Theo W Dreher, W Allen Miller
Jun 9, 2009·Biochimica Et Biophysica Acta·Anne E Simon, Lee Gehrke

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