PMID: 4901706Oct 1, 1969Paper

Repression-dependent alteration of an arginine enzyme in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
T LeisingerH J Vogel

Abstract

Treatment of susceptible Escherichia coli K12 derivatives with 0.4 M Mg(++) at 37 degrees , potentiated by L-arginine or L-canavanine, leads to alteration of acetylornithine delta-transaminase. The alteration, obtained in the absence of protein synthesis and reversible at 0 or 37 degrees , is manifested in extracts by lowered activity and modified substrate affinity behavior of the enzyme without gross changes in sedimentation properties. Cells grown under arginine repression are susceptible to the treatment; cells grown under genetic or steady-state physiological derepression are not. Transaminase synthesized during early derepression can be altered, although to progressively diminishing extents. Enzyme formed under steady-state derepression becomes alterable following transition to repression. The Mg(++) -dependent alteration can be thought to arise while the enzyme, arginine (or canavanine), and aporepressor are in contact, and to reflect a physiological process such as the participation of the enzyme in the repressive complex.

References

Jun 17, 1969·Biochimica Et Biophysica Acta·T Leisinger, H J Vogel
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·A L Cline, R M Bock
May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·S BaumbergH J Vogel
Nov 8, 1965·Biochemical and Biophysical Research Communications·J Bechet, J M Wiame
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·W K MAAS

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Citations

Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·W L McLellan, H J Vogel
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A P Bollon, P T Magee
Jan 1, 1981·Critical Reviews in Microbiology·R A Jensen, D H Calhoun
Jan 20, 2009·Journal of Bacteriology·Catherine S ChanRaymond J Turner
Aug 1, 1972·Journal of Bacteriology·M A SavinC Slaughter
Apr 1, 1976·Journal of Bacteriology·R A Kryzek, P Rogers
Dec 1, 1975·Journal of Bacteriology·V M FernándezA O Ballesteros
Mar 1, 1974·Science·R F Goldberger

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