PMID: 6539768May 1, 1984Paper

Repression of beta-lactam production in Cephalosporium acremonium by nitrogen sources

The Journal of Antibiotics
Y Q ShenA L Demain

Abstract

A variety of inorganic and organic nitrogen sources were added to fermentation media to determine their regulatory effects on the production of beta-lactam antibiotics by Cephalosporium acremonium. (NH4)2SO4 at concentrations higher than 100 mM (1.3%) strongly inhibited beta-lactam production. L-Asparagine and L-arginine proved to be the best nitrogen sources tested for beta-lactam production. The optimum concentration of asparagine was 1.2%. Higher concentrations led to NH3 accumulation, increase in pH, and lower growth rates. Addition of tribasic magnesium phosphate [Mg3(PO4)2 X 8H2O] to the (NH4)2SO4-containing medium stimulated beta-lactam production markedly and ammonium repression of the ring-expansion enzyme was reversed. It appears that the ring-expansion step is a very sensitive part of beta-lactam biosynthesis in C. acremonium with respect to nitrogen source repression. Other enzymes may also be sensitive in view of the fact that nitrogen source derepression not only led to increases in cephalosporin C but, to a lesser extent, penicillin N and total beta-lactam titers.

Citations

Jun 1, 1992·Applied Microbiology and Biotechnology·A LebrihiP Germain
Jan 1, 1996·World Journal of Microbiology & Biotechnology·S S Yang, W J Swei
Jun 1, 1994·Applied Microbiology and Biotechnology·M J Rollins, G M Gaucher
Sep 1, 1995·Canadian Journal of Microbiology·A LounèsP Germain
Dec 3, 2013·Applied Microbiology and Biotechnology·Laura NavoneHugo Gramajo
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·A A Brakhage
Oct 1, 1988·Applied and Environmental Microbiology·M OrvehedK Söderhäll
Dec 21, 1984·Science·S WolfeD W Westlake

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