Repression of invasion genes and decreased invasion in a high-level fluoroquinolone-resistant Salmonella typhimurium mutant.

PloS One
Anna FàbregaJordi Vila

Abstract

Nalidixic acid resistance among Salmonella Typhimurium clinical isolates has steadily increased, whereas the level of ciprofloxacin resistance remains low. The main objective of this study was to characterize the fluoroquinolone resistance mechanisms acquired in a S. Typhimurium mutant selected with ciprofloxacin from a susceptible isolate and to investigate its invasion ability. Three different amino acid substitutions were detected in the quinolone target proteins of the resistant mutant (MIC of ciprofloxacin, 64 microg/ml): D87G and G81C in GyrA, and a novel mutation, E470K, in ParE. A protein analysis revealed an increased expression of AcrAB/TolC and decreased expression of OmpC. Sequencing of the marRAB, soxRS, ramR and acrR operons did not show any mutation and neither did their expression levels in a microarray analysis. A decreased percentage of invasion ability was detected when compared with the susceptible clinical isolate in a gentamicin protection assay. The microarray results revealed a decreased expression of genes which play a role during the invasion process, such as hilA, invF and the flhDC operon. Of note was the impaired growth detected in the resistant strain. A strain with a reverted phenotype (mainly con...Continue Reading

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Citations

Oct 22, 2013·International Journal of Antimicrobial Agents·Clara Ballesté-DelpierreAnna Fàbrega
Apr 8, 2014·The Journal of Antimicrobial Chemotherapy·Anna FàbregaJordi Vila
Nov 13, 2010·Journal of Proteome Research·Iosif VranakisAnna Psaroulaki
Dec 22, 2011·The Journal of Antimicrobial Chemotherapy·Vito RicciLaura J V Piddock
Dec 20, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kwai Lin ThongCindy Shuan Ju Teh
Oct 9, 2015·The Journal of Antimicrobial Chemotherapy·Clara Ballesté-DelpierreJordi Vila
Aug 26, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Fábio CampioniJuliana Pfrimer Falcão
Sep 18, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Felipe Pinheiro VilelaFábio Campioni
Apr 5, 2013·Clinical Microbiology Reviews·Anna Fàbrega, Jordi Vila
Apr 21, 2012·FEMS Microbiology Letters·Anna FàbregaJordi Vila
Jun 8, 2012·The Journal of Veterinary Medical Science·Truong Ha ThaiRyoji Yamaguchi
Jun 27, 2017·The Pediatric Infectious Disease Journal·Emma Sáez-LópezSara M Soto
Aug 5, 2020·The Journal of Antimicrobial Chemotherapy·Chuan-Zhen ZhangHong-Xia Jiang

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Methods Mentioned

BETA
PCR
electrophoresis
Protein Assay

Software Mentioned

Statistical Package for the Social Sciences , SPSS

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