Repression of lipopolysaccharide biosynthesis in Escherichia coli by an antisense RNA of Acetobacter methanolicus phage Acm1

Molecular Microbiology
U MamatG Schmidt

Abstract

Lysogenic Acetobacter methanolicus strains carrying the prophage Acm1 were found to be unable to synthesize both the capsular polysaccharide (CPS) and the O-specific side-chain of lipopolysaccharide (LPS) and to represent rough variants of the host bacterium. A 262 bp DNA fragment of phage Acm1, obviously required for interference with LPS biosynthesis, was cloned and expressed in Escherichia coli. Independently of the O-type, transformation of various E. coli strains with the recombinant DNA resulted in a suppression of biosynthesis of the O-specific chains. The DNA fragment of phage Acm1 contained three very short open reading frames of 21, 24, and 36 bp. However, attempts to express phage-encoded peptides were not successful. Instead, the Acm1-derived DNA fragment was shown to code for the synthesis of a trans-acting RNA molecule of 97 nucleotides, designated lbi (LPS biosynthesis-interfering) RNA. This RNA contains sequence complementarity to E. coli target RNA sequences and appears to have the ability to form intracellularly RNA hybrid duplexes with mRNA. The data presented in this study support the hypothesis that the phenotypic effect of conversion to rough-type LPS is accompanied by the expression of an antisense RNA of...Continue Reading

References

Apr 11, 1975·Infection and Immunity·P GemskiS B Formal
Jan 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·K JannB Jann
Jun 25, 1992·Nucleic Acids Research·M Citron, H Schuster
Oct 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·E UlmerG Schmidt
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A JaegerM Zuker
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Mar 11, 1987·Nucleic Acids Research·C B Harley, R P Reynolds
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jul 1, 1983·Journal of Bacteriology·J Kuzio, A M Kropinski
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T MizunoM Inouye
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jul 1, 1993·Nucleic Acids Research·M KrögerP Rice
Sep 11, 1993·Nucleic Acids Research·S Wang, B J Dolnick

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