Repression of TGF-β Signaling in Breast Cancer Cells by miR-302/367 Cluster

Cell Journal
Mona Ahmadalizadeh KhanehsarArash Javeri

Abstract

Epigenetic alterations of the malignantly transformed cells have increasingly been regarded as an important event in the carcinogenic development. Induction of some miRNAs such as miR-302/367 cluster has been shown to induce reprogramming of breast cancer cells and exert a tumor suppressive role by induction of mesenchymal to epithelial transition, apoptosis and a lower proliferation rate. Here, we aimed to investigate the impact of miR-302/367 overexpression on transforming growth factor-beta (TGF-β) signaling and how this may contribute to tumor suppressive effects of miR-302/367 cluster. In this experimental study, MDA-MB-231 and SK-BR-3 breast cancer cells were cultured and transfected with miR-302/367 expressing lentivector. The impact of miR-302/367 overexpression on several mediators of TGF-β signaling and cell cycle was assessed by quantitative real-time polymerase chain reaction (qPCR) and flow cytometry. Ectopic expression of miR-302/367 cluster downregulated expression of some downstream elements of TGF-β pathway in MDA-MB-231 and SK-BR-3 breast cancer cell lines. Overexpression of miR-302/367 cluster inhibited proliferation of the breast cancer cells by suppressing the S-phase of cell cycle which was in accordance w...Continue Reading

Citations

May 1, 2021·International Journal of Molecular Sciences·Vrinda GoteDhananjay Pal
May 7, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Xian WuLibing Ma

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Methods Mentioned

BETA
PCR
transfection
flow cytometry

Software Mentioned

FlowJo
REST

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