PMID: 9420205Jan 7, 1998Paper

Repression of the A8L gene, encoding the early transcription factor 82-kilodalton subunit, inhibits morphogenesis of vaccinia virions

Journal of Virology
X HuB Moss

Abstract

The vaccinia virus early transcription factor (VETF) is a DNA binding protein comprised of 70- and 82-kDa subunits encoded by the D6R and A8L genes, respectively. A previous investigation suggested a novel role for the 70-kDa subunit in the morphogenesis of vaccinia virus particles. The principal objectives of the present study were to determine if the 82-kDa subunit of VETF is also required for morphogenesis and, if so, whether the block occurs before or after the incorporation of the genome into the assembling virus particle. To address these and other questions, we constructed and characterized a conditionally lethal recombinant vaccinia virus in which the A8L gene is stringently repressed by the Escherichia coli lac operator system. The amount of 82-kDa protein synthesized could be regulated by the amount of inducer: from undetectable to higher than normal levels. Virus replication, as determined by plaque formation or virus yield upon synchronous infection, was dependent on inducer. Nevertheless, de novo synthesis of the 82-kDa subunit was not required for viral early, intermediate, and late gene expression or DNA replication. Overexpression of the A8L gene alone, produced by high concentrations of inducer, inhibited viral...Continue Reading

References

Aug 13, 1976·Science·C Morgan
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B S SchnierleB Moss
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Hagler, S Shuman
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Y Ahn, B Moss
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y F Zhang, B Moss
Apr 1, 1990·Journal of Virology·S S Broyles, B S Fesler
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P D Gershon, B Moss
Jul 1, 1990·Virology·J F Rodriguez, G L Smith
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
May 1, 1988·Virology·R M BullerT Fredrickson
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·G A WardT R Fuerst

❮ Previous
Next ❯

Citations

Aug 15, 2003·The Journal of General Virology·Steven S Broyles
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M D WheelerR G Thurman
Jul 26, 2013·Journal of Virology·P S SatheshkumarBernard Moss
Dec 10, 1999·The Journal of Biological Chemistry·S S BroylesM Kremer
May 11, 1999·Journal of Virology·O WilliamsM Merchlinsky
Jan 5, 2002·Trends in Microbiology·Beate Sodeik, Jacomine Krijnse-Locker
Jun 27, 2002·Virology·S N ShchelkunovB Moss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.