Abstract
This study aimed to investigate the regulatory effects of repressor element-1 silencing transcription factor (REST) on the glutamate receptors and immediate early genes (IEGs) in the SH-SY5Y cells. The genes regulated by REST were screened by bioinformatics between AD patients and the control group. Then, SH-SY5Y cells were treated with 10 μM Aβ or REST siRNA/cDNA, and the expressions of synaptic genes and IEGs were detected. Moreover, the protein expression of synaptophysin and PSD-95 was detected by Western blotting in the primary mouse hippocampal neurons. Firstly, 464 differentially expressed genes regulated by REST were identified between Alzheimer's disease (AD) patients and controls, and REST was closely related to the glutamatergic synapses and long-term potentiation. GRIA1, GRIN2A, GRIN1, and ARC showed significant variations with the changes of REST. Moreover, the loss of REST reduced the expression of synaptophysin and PSD-95, which was related to synaptic plasticity. REST maintains synaptic plasticity by affecting both glutamate receptors and IEGs, and the imbalance between neural excitation and inhibition mediated by REST compromises neural function, contributing to cognitive impairment.
References
Jan 1, 1991·Molecular Neurobiology·W C AbrahamW P Tate
Mar 2, 2010·Neurobiology of Aging·M R PennerC A Barnes
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Min NohR Suzanne Zukin
Dec 3, 2013·Biochimica Et Biophysica Acta·David C BerstenMurray L Whitelaw
Mar 29, 2014·Nature·Tao LuBruce A Yankner
Dec 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Francesco PaonessaFabio Benfenati
Jan 9, 2016·Molecular Neurobiology·Yangang ZhaoJiqiang Zhang
May 26, 2016·Advances in Biological Regulation·Shahram Bahrami, Finn Drabløs
Dec 14, 2016·Journal of Neuroscience Methods·Qian YangYang Tang
Jan 11, 2017·Molecular Psychiatry·A Singh-TaylorT Z Baram
Jan 17, 2017·CNS & Neurological Disorders Drug Targets·Stephen D SkaperPietro Giusti
Apr 20, 2017·Molecular Neurobiology·Ying LiJian-Ming Li
Jun 15, 2017·Brain Structure & Function·Saak V Ovsepian
Sep 25, 2017·Seminars in Cell & Developmental Biology·Hiroyuki OkunoHaruhiko Bito
Mar 22, 2018·Seminars in Cell & Developmental Biology·Irina Epstein, Steven Finkbeiner
Apr 10, 2018·Experimental & Molecular Medicine·Seungjoon KimJi Won Um
May 1, 2018·Neural Plasticity·María Verónica BaezDiana Alicia Jerusalinsky
Jun 5, 2018·Neuron·Mark J WallSonia A L Corrêa
Oct 26, 2018·Neuron·Graham H Diering, Richard L Huganir
Feb 18, 2019·Neuroscience and Biobehavioral Reviews·Alexander V KedrovKonstantin V Anokhin
Apr 21, 2019·Frontiers in Neuroendocrinology·Myrthe Mampay, Graham K Sheridan
Sep 3, 2019·Annals of Translational Medicine·Min FangXueyuan Liu
Oct 18, 2019·Nature·Joseph M ZulloBruce A Yankner
Dec 19, 2019·Frontiers in Cellular Neuroscience·Hey-Kyoung Lee, Alfredo Kirkwood
Jan 8, 2020·International Journal of Molecular Sciences·Jose M Garcia-ManteigaJacopo Meldolesi