Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line

Biological Research
Kurt BucheggerJuan Carlos Roa

Abstract

Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERα(+) tumors showed higher methylation intensity than ERα(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell mig...Continue Reading

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Citations

May 26, 2016·Cancer Letters·Panagiota Economopoulou, Amanda Psyrri
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuanyuan TangYun Liu
Feb 9, 2017·Oncotarget·Esperanza Martín-SánchezDavid Guerrero-Setas
Sep 7, 2019·Journal of Food Biochemistry·Jianli LiuXiangyu Cao
Jun 27, 2018·International Journal of Molecular Sciences·Julio D AmigoAlejandro H Corvalán
Nov 4, 2020·Cell Biology International·Marco A Lacerda-AbreuJosé R Meyer-Fernandes

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Methods Mentioned

BETA
transfection
nuclear translocation
PCR
glycosylation
xenograft
transfections
electrophoresis
protein assay

Software Mentioned

GraphPad Prism
ImageJ
CMS

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