Reprimo (RPRM) is a novel tumor suppressor in pituitary tumors and regulates survival, proliferation, and tumorigenicity.

Endocrinology
Mei XuMargaret E Wierman

Abstract

Reprimo (RPRM), initially identified as a downstream effector of p53-induced cell cycle arrest at G(2)/M, is a putative tumor suppressor silenced in some types of cancer. In microarrays, the RPRM transcript was repressed 26-fold in gonadotrope (null cell) human pituitary tumors compared with normal pituitary but in the absence of changes in p53. Inhibition of RPRM mRNA was confirmed by RT-PCR in all gonadotrope tumors, most GH samples, and variably in other tumor types. Human pituitary tumors showed no evidence of abnormal promoter hypermethylation as a mechanism of RPRM repression. RPRM stable expression in gonadotrope (LβT2) and GH (GH3) pituitary cells resulted in decreased rates of cell proliferation by 55 and 30%, respectively; however, RPRM reexpression did not alter G(2)/M transition. In addition, RPRM increased rates of apoptosis in response to growth factor deprivation as assessed by caspase-3 cleavage and nuclear condensation. Clonagenic assays showed a 5.3- and 3.7-fold suppression of colony growth in RPRM-overexpressing LβT2 and GH3 cells, respectively, supporting its role as a tumor suppressor. In cells stably expressing RPRM mRNA, protein levels were actively suppressed due to rapid degradation through ubiquitinat...Continue Reading

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Citations

Jan 23, 2016·Biological Research·Kurt BucheggerJuan Carlos Roa
Sep 24, 2015·Molecular and Cellular Endocrinology·Katja Kiseljak-VassiliadesMargaret E Wierman
Jan 8, 2014·Endocrinology and Metabolism·Xiaobing Jiang, Xun Zhang
Aug 17, 2016·Oncotarget·Benjamin Garcia-BlojPilar Blancafort
Feb 5, 2015·Molecular Endocrinology·Weipeng XiongMargaret E Wierman
Apr 11, 2018·Frontiers in Neuroanatomy·Karen StanicJulio D Amigo
Jun 14, 2019·Frontiers in Endocrinology·Shehua QianXianquan Zhan
Jun 27, 2018·International Journal of Molecular Sciences·Julio D AmigoAlejandro H Corvalán
Dec 16, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Jiajia Li, Xianquan Zhan
Jul 14, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marc Fernandez-GuerreroMarian A Ros
May 12, 2021·Cancer Discovery·Heng PanDan A Landau

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