PMID: 9418271Jan 7, 1998Paper

Reproducibility and accuracy of interactive segmentation procedures for image analysis in cytology

Journal of Microscopy
A J EinsteinZ Liu

Abstract

The segmentation of nuclear images is a crucial step in the development of procedures using image analysis for the cytological diagnosis of cancer. The purpose of this study is to evaluate the reproducibility and accuracy of several interactive segmentation methods which can be used in this context. Four methods were studied: a thresholding-based method enabling selection of intensity histogram contrast and brightness, manual tracing with a stylus, and arc- and ellipse-fitting routines. Features of nuclear size and shape were derived from nuclei segmented on repeated occasions by several individuals. Variance component models provided a statistical framework for evaluating the intraobserver and interobserver variability of these measurements in terms of their intraclass correlation coefficients. Of the methods tested, the arc-fitting segmentation method gave the most reproducible results, and thresholding the least. Reproducibility was generally very high both between individuals and for repeated segmentations by a single individual. Accuracies of area measurements for the various methods, as determined with respect to point counting, paralleled the reproducibilities of the methods. Sample size requirements were observed to be ...Continue Reading

Citations

Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Rodrigo Fernandez-GonzalezCarlos Ortiz-de-Solórzano
Dec 4, 2003·Medical Image Analysis·Yan KangWilli A Kalender
Oct 20, 2001·Journal of Neuroscience Methods·S S YooP Saiviroonporn
Jan 23, 2004·Cancer Investigation·Joan Gil, Hai-Shan Wu
Jan 14, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J MarekG Dietler
May 19, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Birgitte NielsenHåvard E Danielsen
Jun 4, 2014·Veterinary and Comparative Oncology·R SimeonovM Gulubova
Aug 4, 2004·Micron : the International Research and Review Journal for Microscopy·Noël Bonnet
Oct 10, 2002·Microscopy Research and Technique·Joan GilBeverly Y Wang
Nov 26, 1998·The Journal of Pathology·A J EinsteinJ Gil

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