Reproducibility in measuring tear samples using a freezing point depression osmometer

Clinical & Experimental Optometry : Journal of the Australian Optometrical Association
Hugo Pena-VerdealMaría J Giráldez

Abstract

Hyperosmolarity of tear fluid has been recognised as a common feature of all types of dry eye disease. This study was designed to assess the inter-session reproducibility of a freezing point depression osmometer (Fiske 110) as the most common and precise way of measuring osmolality, by using two different volumes of tear samples on healthy subjects, and to determine the possible applications of this device in tear film research and clinical practice. Measurements were made by using the Fiske 110 osmometer under two different tear sample volumes (4 μl and 2 μl). In both cases, samples were diluted in purified water to obtain the 20 μl required by the device to perform the measurement (1:4 and 1:9 dilutions, respectively). Inter-session reproducibility was determined in two groups of 40 healthy subjects, in two sessions, one week apart. In each group, one of the two different tear sample volumes was used to determine the reproducibility of each technique. No significant differences were detected between the measurements obtained in the two sessions using the 4 μl (paired t-test, p = 0.772; mean difference ± SD = -0.85 ± 18.77 mOsm/L; 95 per cent limits of agreement [LoAs] = -37.64/+35.94) or the 2 μl volume sample (paired t-test,...Continue Reading

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Citations

Nov 15, 2020·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Hugo Pena-VerdealMaría J Giráldez

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