Reproducibility of bacterial and copepod density assessment in bathing and artisanal fishing water of the Eastern Mediterranean

International Journal of Environmental Health Research
Elie BarbourRami Zurayk

Abstract

Faecal bacteriological indicators and copepod density assessment are used to predict the environmental health of seawater for recreational bathing and artisanal fishing, respectively. The reproducibility of bacterial culture-count and copepod-microscopic density assessment after respective capturing of the sampled seawater on 0.22 microm Millipore filter and 150 microm mesh sieve copepod net, is determined. The paired t-test was performed to evaluate the reproducibility of each obtained parameter-mean in first and second simultaneous water samplings of a total of 10 sites selected along a 200 km distance, at about 500-1000 m offshore. The means of each bacterial indicator in colony forming units/100 ml of sea water in first versus (vs.) second sampling of the 10 sites, followed by the P values were: total bacterial count (6.3 x 10(2) vs. 6.2 x 10(2), P=0.958), Coliform count (3.9 x 10(2) vs. 2.6 x 10(2), P=0.212), Staphylococcus aureus (3.0 x 10(2) vs. 2.4 x 10(2), P=0.551), and Clostridium perfringens (1.4 x 10 vs. 0.4 x 10, P=0.298). However, the average copepod density in five microscopic fields at magnification of 100x in sample 1 vs. sample 2 were: (1.40 vs. 1.60 respectively, P=0.267). This sampling design along the 200 k...Continue Reading

References

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