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Reproducibility of Illumina platform deep sequencing errors allows accurate determination of DNA barcodes in cells

BMC Bioinformatics

Apr 4, 2016

Joost B BeltmanTon N M Schumacher

Abstract

Next generation sequencing (NGS) of amplified DNA is a powerful tool to describe genetic heterogeneity within cell populations that can both be used to investigate the clonal structure of cell populations and to perform genetic lineage tracing. For applications in which both abundant an...read more

Mentioned in this Paper

Strategy
Determination Aspects
Nucleic Acid Sequencing
Clone
Sequencing
Massively-Parallel Sequencing
Sequence Determinations, DNA
Deep Sequencing
DNA Barcoding, Taxonomic
High-Throughput Nucleotide Sequencing
16
1
59
Paper Details
References
  • References42
  • Citations3
12345
  • References42
  • Citations3
1

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Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

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Reproducibility of Illumina platform deep sequencing errors allows accurate determination of DNA barcodes in cells

BMC Bioinformatics

Apr 4, 2016

Joost B BeltmanTon N M Schumacher

PMID: 27038897

DOI: 10.1186/s12859-016-0999-4

Abstract

Next generation sequencing (NGS) of amplified DNA is a powerful tool to describe genetic heterogeneity within cell populations that can both be used to investigate the clonal structure of cell populations and to perform genetic lineage tracing. For applications in which both abundant an...read more

Mentioned in this Paper

Strategy
Determination Aspects
Nucleic Acid Sequencing
Clone
Sequencing
Massively-Parallel Sequencing
Sequence Determinations, DNA
Deep Sequencing
DNA Barcoding, Taxonomic
High-Throughput Nucleotide Sequencing
16
1
59

Feeds With Similar Papers

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

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Paper Details
References
  • References42
  • Citations3
12345
  • References42
  • Citations3
1

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