Reproducibility of serum protein profiling by systematic assessment using solid-phase extraction and matrix-assisted laser desorption/ionization mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Anne K CallesenOle N Jensen

Abstract

Protein profiling of human serum by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is potentially a new diagnostic tool for early detection of human diseases, including cancer. Sample preparation is a key issue in MALDI MS and the analysis of complex samples such as serum requires optimized, reproducible methods for handling and deposition of protein samples. Data acquisition in MALDI MS is also a critical issue, since heterogeneity of sample deposits leads to attenuation of ion signals in MALDI MS. In order to improve the robustness and reproducibility of MALDI MS for serum protein profiling we investigated a range of sample preparation techniques and developed a statistical method based on repeated analyses for evaluation of protein-profiling performance of MALDI MS. Two different solid-phase extraction (SPE) methods were investigated, namely custom-made microcolumns and commercially available magnetic beads. Using these two methods, nineteen different sample preparation methods for serum profiling by MALDI MS were systematically tested with regard to matrix selection, stationary phase, selectivity, and reproducibility. Microcolumns were tested with regard to chromatographic properties; reversed phas...Continue Reading

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Citations

Jun 15, 2010·Journal of the American Society for Mass Spectrometry·Simone NicolardiYuri E M van der Burgt
Oct 7, 2010·Pancreas·Edgar Zapico-MuñizJosefina Mora-Brugués
Nov 6, 2009·PloS One·Angelo Gámez-PozoJuan Angel Fresno Vara
Dec 4, 2012·International Journal of Molecular Sciences·Rocco SavinoRosa Terracciano
Oct 22, 2011·Drug Discovery Today·Rocco Savino, Rosa Terracciano
Oct 1, 2014·Analytical Chemistry·Björn HammarströmSimon Ekström
Jan 13, 2009·Advanced Drug Delivery Reviews·Toshiyuki SakaedaKohshi Nishiguchi
Sep 18, 2009·Molecular Nutrition & Food Research·Jasmin MeltretterMonika Pischetsrieder
Jul 31, 2018·Expert Review of Proteomics·Viviana GrecoAndrea Urbani
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
Jun 26, 2021·Life Science Alliance·Lucas Cardoso LazariGiuseppe Palmisano
Apr 19, 2008·Journal of Agricultural and Food Chemistry·Jasmin MeltretterMonika Pischetsrieder

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