Reproducible and controllable light induction of in vitro fruiting of the white-rot basidiomycete Pleurotus ostreatus

Mycological Research
Davinia ArjonaA G Pisabarro

Abstract

Fruiting is a crucial developmental process in basidiomycetes yet the genetic and molecular factors that control it are not yet fully understood. The search for fruiting inducers is of major relevance for both basic research and for their use in industrial applications. In this paper, an efficient and reproducible protocol for controlled fruiting induction of Pleurotus ostreatus growing on synthetic medium is described. The protocol is based on the control of light intensity and photoperiod and permits the life cycle for this fungus to be completed in less than two weeks. The fruiting bodies produced by this method release fertile spores after 4-5 d of culture. Our results indicate that fruiting induction is solely dependent on the illumination regime and that it occurs long before the available nutrients are depleted in the culture. This protocol will greatly facilitate molecular and developmental biology research in this fungus as it avoids the need for complex culture media based on lignocellulosic materials or the use of chemical inducers.

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Citations

Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Yoko NakanoMasanobu Kojima
Aug 28, 2019·Annual Review of Genetics·Luis M Corrochano
Sep 6, 2019·Archives of Microbiology·José Alejandro Sánchez-ArreguinJosé Ruiz-Herrera
Jun 13, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Alessandra BorgognoneLucía Ramírez
Jul 28, 2021·Enzyme and Microbial Technology·Nelma Lopes AraújoJuliana Silveira do Valle
Aug 10, 2021·Ecology and Evolution·Franz-Sebastian KrahClaus Bässler

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