PMID: 9447381Feb 3, 1998Paper

Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis

Histopathology
F H KwaspenF C Ramaekers

Abstract

In this study the recently developed keratin 19 antibody RCK108 is biochemically and immunohistochemically characterized. Its applicability as a keratin marker in routinely processed histological tissue specimens is assessed. The keratin 19 antibody RCK108 antibody was tested on normal and malignant routinely formalin-fixed, paraffin-embedded tissue specimens. It stains most, although not all, glandular epithelia and showed (focal) reactivity in the basal cell compartment of stratified epithelia. It was found to react with most epithelial tumours, including adenocarcinomas, squamous cell carcinomas and endocrine tumours of various origins. Its reproducible and highly sensitive staining characteristics make RCK108 a useful antibody to be applied as a broad epithelial marker for carcinoma detection in routinely processed paraffin sections. As such, RCK108 is a specific reagent for practically all epithelial tumours. A few types of epithelial malignancies, known not to contain keratin 19, were negative for RCK108. Therefore the antibody is also useful in some narrow differential diagnostic considerations such as cholangiocellular carcinoma (RCK108 positive) vs. hepatocellular carcinoma (RCK108 negative). Another important feature ...Continue Reading

Citations

Jun 5, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·Reda S SaadJan F Silverman
Jun 23, 2010·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Frank SmedtsAnton H N Hopman
Aug 2, 2011·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Colin J R StewartKathryn Louwen
Aug 24, 2004·Advances in Anatomic Pathology·Vijay Varma, Cynthia Cohen
Jul 16, 2008·Human Pathology·Patrick A Adegboyega, Suimin Qiu
May 20, 2004·The British Journal of Dermatology·C G GhoF C S Ramaekers
Jan 4, 2001·Gynecologic Oncology·B J QuadeC P Crum
Feb 24, 2004·Acta Oto-laryngologica·Shun-Ichi Imamura, Haruko Suzuki

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