Reproducible discrimination between gram-positive and gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation

The Analyst
Robert PrucekMilan Kolář

Abstract

The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent re-crystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates.

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Citations

Oct 8, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li CuiKaisong Zhang
Apr 22, 2015·Advanced Drug Delivery Reviews·Susanne PahlowJürgen Popp
Jun 26, 2015·Journal of Nanobiotechnology·Longyan ChenSuresh Neethirajan
Jun 9, 2017·Journal of Clinical Microbiology·Meron GhebremedhinNicole J Crane
Aug 24, 2017·Scientific Reports·Aeli P OlsonNathan C Lindquist
Jun 21, 2017·Scientific Reports·Shirly BerezinYaakov R Tischler
Jan 23, 2021·Applied Microbiology and Biotechnology·Shuaishuai YanQing Liu

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