Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells.

Frontiers in Immunology
Anne L FletcherShannon J Turley

Abstract

Within lymph nodes, non-hematopoietic stromal cells organize and interact with leukocytes in an immunologically important manner. In addition to organizing T and B cell segregation and expressing lymphocyte survival factors, several recent studies have shown that lymph node stromal cells shape the naïve T cell repertoire, expressing self-antigens which delete self-reactive T cells in a unique and non-redundant fashion. A fundamental role in peripheral tolerance, in addition to an otherwise extensive functional portfolio, necessitates closer study of lymph node stromal cell subsets using modern immunological techniques; however this has not routinely been possible in the field, due to difficulties reproducibly isolating these rare subsets. Techniques were therefore developed for successful ex vivo and in vitro manipulation and characterization of lymph node stroma. Here we discuss and validate these techniques in mice and humans, and apply them to address several unanswered questions regarding lymph node composition. We explored the steady-state stromal composition of lymph nodes isolated from mice and humans, and found that marginal reticular cells and lymphatic endothelial cells required lymphocytes for their normal maturation...Continue Reading

Citations

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Datasets Mentioned

BETA
GSE15907

Methods Mentioned

BETA
flow cytometry
FACS
Assay
chip
FCS

Software Mentioned

Depletes
DAVID Annotation
Expression File Creator
DAVID
Prism
FACSDiva
GenePattern
Excel
Multiplot

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