Reproductive condition and the low-dose endotoxin-induced inflammatory response in rats. Glomerular influx of inflammatory cells and expression of adhesion molecules

Biology of Reproduction
M M FaasG A Schuiling

Abstract

These experiments were designed to study the increased sensitivity of pregnant rats to endotoxin. Pregnant (Pr), cyclic (C), and progesterone (P)-treated pseudopregnant rats with or without a decidualized uterus (PSP and DEC rats, respectively) received infusions of an ultra-low dose of endotoxin (1.0 microg/kg BW) and were killed 3 days later. Pr, PSP, and DEC rats were infused on Day 14, C rats on diestrus. Endotoxin-infused rats were compared with saline-infused rats in the same reproductive conditions. The inflammatory reaction of the glomeruli of the kidneys was studied by immunohistochemical methods using 4-microm cryostat sections stained with specific monoclonal antibodies against neutrophils (polymorphonuclear cells, PMNs) and monocytes (MOs), and against the adhesion molecules ICAM-1 and VCAM-1 on the endothelium, and LFA-1, MAC-1, and VLA-4 on the leukocytes. Endotoxin infusion increased glomerular PMN and MO number in Pr, PSP, and DEC rats, all of which have elevated P levels, but not in C rats, which do not. The endotoxin-induced expression of adhesion molecules, associated with this influx of inflammatory cells, varied with the reproductive condition. In C rats there was no increased adhesion molecule expression a...Continue Reading

Citations

Aug 23, 2002·Current Opinion in Rheumatology·Joan T Merrill
Sep 7, 2000·Obstetrical & Gynecological Survey·L CarbillonS Uzan
May 18, 1999·American Journal of Reproductive Immunology : AJRI·M M FaasG A Schuiling
Nov 15, 2000·Journal of Psychosomatic Obstetrics and Gynaecology·G A Schuiling
Feb 29, 2000·American Journal of Obstetrics and Gynecology·M M FaasC W Redman
Jun 24, 2004·American Journal of Reproductive Immunology : AJRI·Marijke M FaasPaul de Vos

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