Reproductive deficiencies in transgenic mice expressing the rat inhibin alpha-subunit gene

Endocrinology
B N ChoKelly E Mayo

Abstract

Inhibin is an important modulator of reproductive function at both the endocrine level, through its regulation of pituitary FSH biosynthesis, and at the paracrine and autocrine levels, as an intragonadal regulatory factor. To investigate the in vivo actions of inhibin in FSH regulation and gonadal function, transgenic mice that overexpress the rat inhibin alpha-subunit gene were generated. A transgene that includes the mouse metallothionein-I gene promoter (MT-alpha) fused to the rat inhibin alpha-subunit precursor coding sequences was used to produce three lines of transgenic mice. Transgene mRNA is expressed in numerous tissues, including the pituitary, liver, testis, ovary, and kidney. Inhibin alpha-subunit protein was also increased in transgenic pituitary and ovary. Serum inhibin alpha-subunit levels are highly increased compared with control mice. Inhibin beta(A)- and beta(B)-subunit protein amounts are lower in transgenic ovaries compared with wild type, although serum levels of activin A are not significantly reduced in transgenic female mice. FSH levels are reduced in both male and female transgenic mice, whereas LH levels are increased in MT-alpha female mice. MT-alpha transgenic females are subfertile and exhibit a 5...Continue Reading

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Citations

Jan 17, 2013·Molecular Endocrinology·Zhilin LiuJoanne S Richards
Oct 31, 2009·The International Journal of Developmental Biology·Eugene D Albrecht, Gerald J Pepe
May 20, 2005·Cell and Tissue Research·Barbara C Vanderhyden
Nov 27, 2015·Molecules and Cells·Mi-Nyeu KimByung-Nam Cho
Jan 20, 2006·Molecular and Cellular Endocrinology·Gerald J PepeEugene D Albrecht
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May 15, 2012·International Journal of Oncology·Young Il KimByung-Nam Cho
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Jul 25, 2021·General and Comparative Endocrinology·Lu HuangShaojun Liu
Nov 26, 2021·Frontiers in Veterinary Science·Xue YuAixin Liang

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