Reprogramming diminishes retention of Mycobacterium leprae in Schwann cells and elevates bacterial transfer property to fibroblasts

F1000Research
Toshihiro MasakiAnura Rambukkana

Abstract

Bacterial pathogens can manipulate or subvert host tissue cells to their advantage at different stages during infection, from initial colonization in primary host niches to dissemination. Recently, we have shown that Mycobacterium leprae (ML), the causative agent of human leprosy, reprogrammed its preferred host niche de-differentiated adult Schwann cells to progenitor/stem cell-like cells (pSLC) which appear to facilitate bacterial spread. Here, we studied how this cell fate change influences bacterial retention and transfer properties of Schwann cells before and after reprogramming. Using primary fibroblasts as bacterial recipient cells, we showed that non-reprogrammed Schwann cells, which preserve all Schwann cell lineage and differentiation markers, possess high bacterial retention capacity when co-cultured with skin fibroblasts; Schwann cells failed to transfer bacteria to fibroblasts at higher numbers even after co-culture for 5 days. In contrast, pSLCs, which are derived from the same Schwann cells but have lost Schwann cell lineage markers due to reprogramming, efficiently transferred bacteria to fibroblasts within 24 hours. ML-induced reprogramming converts lineage-committed Schwann cells with high bacterial retention ...Continue Reading

References

Dec 1, 1988·Journal of the Neurological Sciences·V P ShettyJ M Jacobs
Mar 10, 2001·Nature·S T ColeB G Barrell
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nikos Tapinos, Anura Rambukkana
Mar 8, 2007·Annual Review of Neuroscience·Zu-Lin ChenSidney Strickland
Apr 21, 2010·The Journal of Infectious Diseases·Ramanuj LahiriJames L Krahenbuhl
Oct 26, 2010·Journal of Visualized Experiments : JoVE·Andrei SeluanovVera Gorbunova
May 28, 2011·The Lancet Infectious Diseases·Laura C Rodrigues, Diana Nj Lockwood
Dec 24, 2011·Trends in Neurosciences·Jorge A PereiraUeli Suter
Oct 26, 2012·Journal of Neuropathology and Experimental Neurology·Laurence RichardJean-Michel Vallat
Mar 8, 2013·Advances in Experimental Medicine and Biology·Lalita Ramakrishnan

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Citations

Dec 30, 2014·Current Opinion in Microbiology·Samuel Hess, Anura Rambukkana
Feb 11, 2015·Expert Review of Clinical Immunology·Priscila Ribeiro AndradeEuzenir Nunes Sarno
Jun 14, 2016·Biochemistry. Biokhimii︠a︡·P V ZolotukhinV A Chistyakov
Mar 13, 2021·Immunological Reviews·Anouk van Hooij, Annemieke Geluk
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Lynn NazarethJenny Ekberg
Jul 20, 2019·Microbiology Spectrum·Samuel Hess, Anura Rambukkana

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Methods Mentioned

BETA
FACS
FCS
chips

Software Mentioned

Bioconductor Analysis suite
SigmaPlot
R

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