May 8, 2015

Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature

PLoS Genetics
Samantha M ThomasYoav Gilad

Abstract

Renewable in vitro cell cultures, such as lymphoblastoid cell lines (LCLs), have facilitated studies that contributed to our understanding of genetic influence on human traits. However, the degree to which cell lines faithfully maintain differences in donor-specific phenotypes is still debated. We have previously reported that standard cell line maintenance practice results in a loss of donor-specific gene expression signatures in LCLs. An alternative to the LCL model is the induced pluripotent stem cell (iPSC) system, which carries the potential to model tissue-specific physiology through the use of differentiation protocols. Still, existing LCL banks represent an important source of starting material for iPSC generation, and it is possible that the disruptions in gene regulation associated with long-term LCL maintenance could persist through the reprogramming process. To address this concern, we studied the effect of reprogramming mature LCL cultures from six unrelated donors to iPSCs on the ensuing gene expression patterns within and between individuals. We show that the reprogramming process results in a recovery of donor-specific gene regulatory signatures, increasing the number of genes with a detectable donor effect by a...Continue Reading

Mentioned in this Paper

Neuro-Oncological Ventral Antigen 2
Thaw
Real-Time Polymerase Chain Reaction
Endoderm
Study
Patterns
Transfection
Specimen Type - Fibroblasts
Pluripotent Stem Cells
Exons

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