Reprogramming of Chromatin Accessibility in Somatic Cell Nuclear Transfer Is DNA Replication Independent

Cell Reports
Mohamed Nadhir DjekidelYi Zhang

Abstract

Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNase I hypersensitive sites (DHSs) in donor cumulus cells and one-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hr. Interestingly, this DHS reprogramming takes place even in the presence of a DNA replication inhibitor, suggesting that SCNT-mediated DHS reprogramming is independent of DNA replication. Thus, this study not only reveals the rapid and drastic nature of the changes in chromatin accessibility through SCNT but also establishes a DNA replication-independent model for studying cellular reprogramming.

Citations

Oct 17, 2019·Cold Spring Harbor Perspectives in Biology·Melanie D White, Nicolas Plachta
Feb 13, 2019·The Journal of Reproduction and Development·Kei Miyamoto
Jul 17, 2020·Protein & Cell·Ruimin XuShaorong Gao
Jan 23, 2021·EMBO Reports·Dongwei LiDuanqing Pei
Jan 1, 2021·International Journal of Molecular Sciences·Kilian SimmetValeri Zakhartchenko
Apr 24, 2021·The FEBS Journal·Junko Tomikawa, Kei Miyamoto
Jun 1, 2021·Current Opinion in Genetics & Development·Eva Hörmanseder

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
ChIP-seq
RNA-seq
PCA

Software Mentioned

HOMER
ZGA
GREAT
R

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Related Papers

Theriogenology
N Rodriguez-OsorioE Memili
The International Journal of Developmental Biology
Chanchao LorthongpanichChin Yan Lim
Reproduction : the Official Journal of the Society for the Study of Fertility
L C BuiV Duranthon
© 2022 Meta ULC. All rights reserved