Reprogramming of gene expression in Escherichia coli cultured on pyruvate versus glucose

Molecular Genetics and Genomics : MGG
Anna Chao KaberdinaVladimir R Kaberdin

Abstract

Previous studies revealed important roles of small RNAs (sRNAs) in regulation of bacterial metabolism, stress responses and virulence. However, only a minor fraction of sRNAs is well characterized with respect to the spectra of their targets, conditional expression profiles and actual mechanisms they use to regulate gene expression to control particular biological pathways. To learn more about the specific contribution of sRNAs to the global regulatory network controlling the Escherichia coli central carbon metabolism (CCM), we employed microarray analysis and compared transcriptome profiles of E. coli cells grown on two alternative minimal media supplemented with either pyruvate or glucose, respectively. Microarray analysis revealed that utilization of these alternative carbon sources led to profound differences in gene expression affecting all major gene clusters associated with CCM as well as expression of several known (CyaR, RyhB, GcvB and RyeA) and putative (C0652) sRNAs. To assess the impact of transcriptional reprogramming of gene expression on E. coli protein abundance, we also employed two-dimensional protein gel electrophoresis. Our experimental data made it possible to determine the major pathways for pyruvate assim...Continue Reading

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Citations

May 26, 2021·NPJ Biofilms and Microbiomes·M Fata Moradali, Mary E Davey

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Datasets Mentioned

BETA
E-MTAB-5432

Methods Mentioned

BETA
electrophoresis
Feature Extraction
chip

Software Mentioned

LIMMA
[UNK] 2D Platinum
WebMeV ( Multiple Experiment Viewer
FlexControl
Feature Extraction
STRING
GeneSpring MultiOmic Analysis
Mascot
Analysis
Biotools

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