Reprogramming of HUVECs into induced pluripotent stem cells (HiPSCs), generation and characterization of HiPSC-derived neurons and astrocytes

PloS One
Yohannes HaileNadia Jahroudi

Abstract

Neurodegenerative diseases are characterized by chronic and progressive structural or functional loss of neurons. Limitations related to the animal models of these human diseases have impeded the development of effective drugs. This emphasizes the need to establish disease models using human-derived cells. The discovery of induced pluripotent stem cell (iPSC) technology has provided novel opportunities in disease modeling, drug development, screening, and the potential for "patient-matched" cellular therapies in neurodegenerative diseases. In this study, with the objective of establishing reliable tools to study neurodegenerative diseases, we reprogrammed human umbilical vein endothelial cells (HUVECs) into iPSCs (HiPSCs). Using a novel and direct approach, HiPSCs were differentiated into cells of central nervous system (CNS) lineage, including neuronal, astrocyte and glial cells, with high efficiency. HiPSCs expressed embryonic genes such as nanog, sox2 and Oct-3/4, and formed embryoid bodies that expressed markers of the 3 germ layers. Expression of endothelial-specific genes was not detected in HiPSCs at RNA or protein levels. HiPSC-derived neurons possess similar morphology but significantly longer neurites compared to prim...Continue Reading

References

Nov 30, 1990·Biochemical and Biophysical Research Communications·M HashimotoM Muramatsu
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D P YounkinD Pleasure
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D A Dawson, J M Hallenbeck
Jun 20, 1997·Brain Research·Y IwahoriN Nishiyama
Jun 27, 1998·Biochemical and Biophysical Research Communications·Z Y GaoB A Wolf
Jan 1, 1999·Journal of Neurotrauma·K E SaatmanT K McIntosh
Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D M KerkovichJ Busciglio
Apr 12, 2000·Journal of Neuropathology and Experimental Neurology·S X ZhangJ W Geddes
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ajit J ShahChristian Heidbreder
Jun 11, 2003·The Journal of Cell Biology·Prithi RajanRonald D G McKay
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabrizio GiulianiV Wee Yong
May 29, 2004·Trends in Cognitive Sciences·Albert Costa, Mikel Santesteban
Nov 10, 2004·Neurology·J M ShefnerUNKNOWN NEALS Consortium
May 17, 2005·Biochemical and Biophysical Research Communications·Alexander K MurashovLaxmansa C Katwa
Aug 27, 2005·Oncogene·Alison C Varga, Jeffrey L Wrana
Jul 25, 2006·Nature Methods·Tenneille E LudwigJames A Thomson
Aug 8, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Suzanne L GrantGlen B Baker
Nov 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Araya RuangkittisakulKlaus Ballanyi
Nov 22, 2007·Science·Junying YuJames A Thomson
Jul 18, 2008·Transplantation Reviews·Zachary J Kastenberg, Jon S Odorico
Jul 23, 2008·Perspectives in Vascular Surgery and Endovascular Therapy·Kimon Bekelis, Nicos Labropoulos
Mar 17, 2009·Experimental & Molecular Medicine·Byoung-San MoonKang-Yell Choi
Apr 3, 2009·Brain : a Journal of Neurology·Josa M FrischerHans Lassmann
Aug 5, 2009·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A V EremeevM A Lagarkova
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ning SunJoseph C Wu
Oct 15, 2009·Nature Biotechnology·Elayne M ChanThorsten M Schlaeger
Jan 26, 2010·Nature Reviews. Immunology·Bart O Roep, Mark Peakman
Mar 17, 2010·Biochemical and Biophysical Research Communications·Kotaro SuzukiMasahiko Ikekita
Mar 31, 2010·Nature Biotechnology·Debora SteinerBenjamin Reubinoff
May 21, 2010·PLoS Computational Biology·Ronald A J van Elburg, Arjen van Ooyen

❮ Previous
Next ❯

Citations

Apr 11, 2017·Molecular Genetics and Metabolism·D TavianD A Coviello
Jun 28, 2018·American Journal of Physiology. Cell Physiology·Kaela M VarbergLaura S Haneline

❮ Previous
Next ❯

Datasets Mentioned

BETA
IPS5449
GSE48257
GSE46397

Methods Mentioned

BETA
PCR
Chip
fluorescence microscopy
protein assay
confocal microscopy
flow cytometry

Software Mentioned

FluoView
GraphPad Prism
GeneSpring
Affymetrix Primeview Chip

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Astrocytes in Parkinson Disease

Parkinson's disease (PD) is a neurodegenerative disorder caused by the progressive loss of dopaminergic neurons. Some PD-genes may be associated with astrocyte dysfunction. Discover the latest research on astrocytes in Parkinson's disease here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Alzheimer's Disease: RNA Regulation

RNA regulation involves several mechanisms that are used by cells to decrease or increase the production of RNA. Disruption of RNA regulatory processes has been associated with Alzheimer's disease (AD). Here are the latest discoveries pertaining to RNA regulation and AD.

Astrocytes & Huntington’s Disease

Astrocytes are abundant within the central nervous system and their dysfunction has been thought to be an important contributor to some neurodegenerative diseases, in particular Huntington’s disease. Damage to these cells may make neurons more susceptible to degeneration. Here is the latest research on astrocytes and Huntington’s disease.

Astrocytes & Huntington’s Disease (MDS)

Astrocytes are abundant within the central nervous system and their dysfunction has been thought to be an important contributor to some neurodegenerative diseases, in particular Huntington’s disease. Damage to these cells may make neurons more susceptible to degeneration. Here is the latest research on astrocytes and Huntington’s disease.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

ALS: Pathogenic Mechanisms

Amyotrophic Lateral Sclerosis is a progressive neurodegenerative disorder characterized by muscle weakness. Here is the latest research investigating pathogenic mechanisms that underlie this genetically heterogeneous disorder.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.