PMID: 9532789Apr 9, 1998Paper

Reptile lysozyme: the complete amino acid sequence of soft-shelled turtle lysozyme and its activity

Bioscience, Biotechnology, and Biochemistry
T ArakiT Torikata

Abstract

Soft-shelled turtle egg-white lysozyme was purified and sequenced. Lysozyme was reduced and carboxymethylated to fragment it with trypsin, V8 protease and CNBr. The peptides yielded were purified by RP-HPLC and sequenced. Every trypsin peptide was overlapped by V8 protease peptides and CNBr fragment. The amino acid sequence was compared with other lysozymes. This lysozyme has an extra Gly residue at N-terminus, which was found in pheasant lysozyme. Further, this lysozyme has an insertion of a Gly residue between 47 and 48 residues when compared with chicken lysozyme, as found in human lysozyme, therefore it proved that this lysozyme has the largest number of amino acids (131 aa) in chicken type lysozymes. The amino acid substitutions were found at subsites E and F. Namely Phe34, Arg45, Thr47, and Arg114 were replaced by His, Tyr, Arg, and Tyr, respectively. The time course using N-acetylglucosamine pentamer as a substrate showed a reduction of the rate constant for glycosidic cleavage and increase of binding free energy for subsites E and F, which proved the contribution of amino acids mentioned above for substrate binding at subsites E and F.

References

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Citations

Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Shunsuke KawamuraTomohiro Araki
Apr 10, 2012·Bioscience, Biotechnology, and Biochemistry·Shunsuke KawamuraTomohiro Araki
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Shunsuke KawamuraTakao Torikata
Apr 3, 2004·Folia Microbiologica·P SímaK Sigler
Jun 12, 2014·Pharmaceuticals·Monique L van Hoek
Sep 15, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Pornpimol PonkhamSompong Thammasirirak
Mar 3, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sompong ThammasirirakJisnuson Svasti
Mar 22, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sompong ThammasirirakJisnuson Svasti
Nov 29, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yu ZhaoYun Zhang
Sep 2, 2006·The Protein Journal·Yuki ChijiiwaTomohiro Araki
Sep 2, 2006·The Protein Journal·Somporn KatekaewTomohiro Araki
Jan 4, 2007·The Protein Journal·Somporn KatekaewTomohiro Araki
Dec 4, 1998·Bioscience, Biotechnology, and Biochemistry·T ArakiT Torikata
Apr 24, 2010·Journal of Biosciences·Lien Callewaert, Chris W Michiels
Feb 28, 2002·Bioscience, Biotechnology, and Biochemistry·Sompong ThammasirirakTomohiro Araki
Feb 16, 2010·The Journal of Experimental Biology·L M ZimmermanR M Bowden

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