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Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

Cell

Mar 5, 2013

Lei S QiWendell A Lim

Abstract

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a ca...read more

Mentioned in this Paper

Pathologic Cytolysis
Establishment and Maintenance of Localization
Zinc Fingers
Flow Cytometry
Deoxyribonuclease I
Transfection
HEK293 Cells
Transcription Repressor/Corepressor
Alkalescens-Dispar Group
Gene Knockdown Techniques
97
7
6
5149
1
1
Paper Details
References
  • References23
  • Citations870
123
  • References23
  • Citations870
12345...

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© 2020 Meta ULC. All rights reserved

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

Cell

Mar 5, 2013

Lei S QiWendell A Lim

PMID: 23452860

DOI: 10.1016/j.cell.2013.02.022

Abstract

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a ca...read more

Mentioned in this Paper

Pathologic Cytolysis
Establishment and Maintenance of Localization
Zinc Fingers
Flow Cytometry
Deoxyribonuclease I
Transfection
HEK293 Cells
Transcription Repressor/Corepressor
Alkalescens-Dispar Group
Gene Knockdown Techniques
97
7
6
5149
1
1

Similar Papers Found In These Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Perturb-seq

Perturb-seq has combined CRISPR gene inactivation and single-cell rna-sequencing (scRNA-seq) and is the newest addition to the geneticist's arsenal, providing scientists with study methods for functional genomics. Discover the latest research here.

Related Papers

Ernst Schering Research Foundation Workshop

RNAi applications in target validation

Ernst Schering Research Foundation WorkshopJanuary 26, 2007
A KourtidisD S Conklin
Paper Details
References
  • References23
  • Citations870
123
  • References23
  • Citations870
12345...
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