Repurposing of clinically approved drugs for treatment of coronavirus disease 2019 in a 2019-novel coronavirus-related coronavirus model.

Chinese Medical Journal
Hua-Hao FanYi-Gang Tong

Abstract

Medicines for the treatment of 2019-novel coronavirus (2019-nCoV) infections are urgently needed. However, drug screening using live 2019-nCoV requires high-level biosafety facilities, which imposes an obstacle for those institutions without such facilities or 2019-nCoV. This study aims to repurpose the clinically approved drugs for the treatment of coronavirus disease 2019 (COVID-19) in a 2019-nCoV-related coronavirus model. A 2019-nCoV-related pangolin coronavirus GX_P2V/pangolin/2017/Guangxi was described. Whether GX_P2V uses angiotensin-converting enzyme 2 (ACE2) as the cell receptor was investigated by using small interfering RNA (siRNA)-mediated silencing of ACE2. The pangolin coronavirus model was used to identify drug candidates for treating 2019-nCoV infection. Two libraries of 2406 clinically approved drugs were screened for their ability to inhibit cytopathic effects on Vero E6 cells by GX_P2V infection. The anti-viral activities and anti-viral mechanisms of potential drugs were further investigated. Viral yields of RNAs and infectious particles were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and plaque assay, respectively. The spike protein of coronavirus GX_P2V shares 92.2% amino acid ...Continue Reading

References

May 24, 2011·Pharmacological Reports : PR·Moshe Rogosnitzky, Rachel Danks
Oct 10, 2013·Chinese Journal of Cancer·Rishil J KathawalaZhe-Sheng Chen
May 21, 2014·Antimicrobial Agents and Chemotherapy·Julie DyallMatthew B Frieman

❮ Previous
Next ❯

Citations

Feb 26, 2020·Journal of Clinical Medicine·Péter BoldogGergely Röst
Mar 4, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Tracy H T LaiKenneth K W Li
Mar 19, 2020·Cadernos de saúde pública·Raquel Martins LanaCláudia Torres Codeço
Mar 19, 2020·International Journal of Clinical Practice·George Thomson
Apr 28, 2020·Seminars in Cardiothoracic and Vascular Anesthesia·Linda Y Tang, Jingping Wang
Apr 30, 2020·Phytotherapy Research : PTR·Solomon HabtemariamSeyed Mohammad Nabavi
May 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rami A BalloutAlan T Remaley
May 20, 2020·Journal of Clinical Medicine·Umesh KalathiyaJavier Antonio Alfaro
May 26, 2020·Journal of Biomolecular Structure & Dynamics·Osmair Vital de OliveiraLuciano T Costa
Apr 12, 2020·Clinical Rheumatology·Durga Prasanna MisraOlena Zimba
Jul 18, 2020·Journal of Biomolecular Structure & Dynamics·Shruti KoulgiRajendra Joshi
Jul 16, 2020·Pathogens and Disease·Zhilong JiaKunlun He
Sep 6, 2020·Pharmacological Reports : PR·Thakur Uttam SinghRaj Kumar Singh
Apr 19, 2020·Clinical Medicine : Journal of the Royal College of Physicians of London·Beattie Rh Sturrock, Timothy Jt Chevassut
Oct 1, 2020·Molecular Diversity·Seyed Aghil HooshmandAli Masoudi-Nejad
Apr 10, 2020·Viruses·Claudia CavaIsabella Castiglioni
Jul 29, 2020·Frontiers in Physiology·Francisco J Barrantes
May 14, 2020·Journal of Experimental & Clinical Cancer Research : CR·Gennaro CilibertoMarco G Paggi
Aug 21, 2020·Cell Death & Disease·Allan SauvatGuido Kroemer
Jul 28, 2020·Journal of Biomolecular Structure & Dynamics·Rohitash YadavShailendra Handu
Jun 18, 2020·Viruses·Aleksandr IanevskiDenis E Kainov
Sep 4, 2020·Annals of Clinical Microbiology and Antimicrobials·Ali A RabaanHakan Leblebicioglu
Aug 29, 2020·Cells·Muhammad Aslam, Yury Ladilov
Jul 24, 2020·Pharmacological Reports : PR·Moshe RogosnitzkyIgor Koman
Nov 5, 2020·Briefings in Bioinformatics·Serena DotoloRoberto Tagliaferri
Jan 11, 2021·Briefings in Bioinformatics·Shasha LiChenyang Lu
Dec 22, 2020·Bioinformatics·Astghik SargsyanMartin Hofmann-Apitius
Jan 24, 2021·Medicinal Research Reviews·Cátia Santos-PereiraManuela Côrte-Real
Jan 26, 2021·Journal of Biomolecular Structure & Dynamics·Avinash Mishra, Anurag S Rathore
Oct 6, 2020·Journal of Drug Delivery Science and Technology·Maheswari NagendranVelayudhaperumal Chellam Padmanaban
Jan 12, 2021·BioMed Research International·Tapan Kumar MohantaPaola Defilippi
Oct 30, 2020·Infection and Drug Resistance·Raphael B Stricker, Melissa C Fesler
Nov 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mathieu GendrotBruno Pradines

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Reviews. Drug Discovery
Guangdi Li, Erik De Clercq
American Journal of Respiratory and Critical Care Medicine
W Graham CarlosShazia Jamil
© 2021 Meta ULC. All rights reserved