PMID: 7581150Aug 1, 1995Paper

Requirement for B cells in T cell priming to minor histocompatibility antigens and development of graft-versus-host disease

Bone Marrow Transplantation
Kirk R SchultzK T HayGlass

Abstract

Increased understanding of minor histocompatibility complex (MiHC) antigen presentation to donor T cells may permit methods to modulate graft-versus-host disease (GVHD), a major complication of allogeneic bone marrow transplantation (BMT). Previously, we described the importance of B cells as antigen presenting cells in T cell responses to a virally induced murine leukemia. Using a B cell deficient mouse model in which mice receive either control rabbit immunoglobulin (RIgG) or rabbit anti-IgM mu chain from birth (B cell deficient), we evaluated whether B cells were necessary for T cell responses to MiHC and the induction of GHVD. Normal and B cell deficient C57BL/6 (H-2b) mice were primed with BALB.B (H-2b; MiHC incompatible) spleen cells and evaluated > 4 weeks later in vitro. While splenic or lymph node T cells obtained from BALB.B primed control C57BL/6 mice demonstrated strong in vitro proliferative responses to MiHC mismatched targets, B cell deficient hosts were markedly reduced to 14-42% of controls. Similarly, a strong MiHC specific cytolytic T cell response was observed in control C57BL/6 mice (53-100% specific cytotoxicity) whereas B cell depleted recipients had no activity (< or = 5% specific lysis). The role of B c...Continue Reading

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