Requirement for bivalent cations in the actions of insulin and sodium nitroprusside on metabolism in rat adipocytes

FEBS Letters
S Emami, M C Perry

Abstract

The requirement for Ca2+ and Mg2+ in the actions of insulin and sodium nitroprusside on rat adipocyte metabolism was investigated: sodium nitroprusside, but not insulin, increased cGMP levels in cells incubated in the absence of Ca2+ and/or Mg2+; sodium nitroprusside and insulin are unable to increase the incorporation of [14C]glucose into triglycerides and [14C]leucine into proteins in the absence of Ca2+ and Mg2+; sodium nitroprusside and insulin showed antilipolytic actions in Ca2+- and Mg2+-free medium. We conclude that in the absence of Ca2+ and Mg2+, sodium nitroprusside and insulin have very similar regulatory properties on triglyceride, protein synthesis and adrenaline-stimulated lipolysis, but not on cGMP levels in rat adipocytes. This could provide evidence that omission of bivalent cations was inhibitory at more than one site, or that sodium nitroprusside mimics insulin's actions by another mechanism that does not involve cGMP.

References

Nov 10, 1975·Biochimica Et Biophysica Acta·R A Akhtar, M C Perry
Nov 29, 1973·Biochimica Et Biophysica Acta·R J Schimmel
May 1, 1974·Endocrinology·R J Schimmel
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G IllianoP Cuatrecasas
Jan 1, 1974·Acta Endocrinologica. Supplementum·T ClausenB R Martin
Nov 24, 1972·Biochimica Et Biophysica Acta·J GliemannS Gammeltoft
Sep 7, 1973·Biochimica Et Biophysica Acta·B O Jacobs, M E Krahl
Dec 8, 1962·Nature·P B GARLAND, P J RANDLE

Related Concepts

Fat Pad
Metazoa
Calcium
Cations, Divalent
Ferricyanides
Anhydrous Dextrose
Cyclic GMP
Insulin B Chain
Lipolysis
Magnesium

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