Requirement for G protein activity at a specific time during embryonic chick myogenesis

Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists
R E HausmanF Craft

Abstract

Signaling between embryonic myoblasts involves prostaglandin metabolism, the activation of a membrane receptor and changes in polyphosphatidyl inositol metabolism. Many of these membrane-localized events occur between 33 to 35 h of differentiation, concomitant with a dramatic change in membrane organization, in myoblast aggregates in culture. Since many receptors affect inositol phosphate metabolism by activating a GTP-binding protein (G protein), we asked if there was evidence for such a protein in myogenic signaling. We show that during the period of differentiation in culture when prostaglandin is needed to bind to a transient receptor, a pertussis toxin-sensitive but cholera toxin-insensitive G protein must act. If this activation is blocked, the characteristic change in myoblast cell adhesion and subsequent membrane fusion do not occur. We suggest that a G protein couples the activated prostaglandin receptor and the change in polyphosphatidyl inositol metabolism and that this membrane transduction step is necessary for subsequent membrane differentiation events during myogenesis.

References

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Citations

Dec 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·H Elgendy, R E Hausman
Nov 30, 2016·Journal of Cachexia, Sarcopenia and Muscle·Christopher Lipina, Harinder S Hundal

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